AIM: We report an in vitro technique to establish alignment of collagen fibres and cells within a three-dimensional tissue equivalent that mimics the natural periodontal ligament (PDL) using a novel custom-designed bioreactor. MATERIAL AND METHODS: Shear stress was applied to the tissue equivalent prepared with collagen solution and seeded with human PDL cells. Stress-strain dynamics and the alignment of collagen fibres and PDL cells in tissue equivalents were analysed. RESULTS: Shear stress aligned collagen fibres and PDL cells in a direction parallel to the principle strain vector. PDL cells and Collagen fibres aligned in strained tissue equivalents with higher uniformity than in unstrained tissue equivalents. CONCLUSIONS: The cell and fibre alignment of the engineered PDL was precisely guided by mechanical shear stress along the direction of principal strain vector using a custom-designed bioreactor, suggesting that the enhanced functional property of engineered PDL constructs could be achieved with this technique.
AIM: We report an in vitro technique to establish alignment of collagen fibres and cells within a three-dimensional tissue equivalent that mimics the natural periodontal ligament (PDL) using a novel custom-designed bioreactor. MATERIAL AND METHODS: Shear stress was applied to the tissue equivalent prepared with collagen solution and seeded with human PDL cells. Stress-strain dynamics and the alignment of collagen fibres and PDL cells in tissue equivalents were analysed. RESULTS: Shear stress aligned collagen fibres and PDL cells in a direction parallel to the principle strain vector. PDL cells and Collagen fibres aligned in strained tissue equivalents with higher uniformity than in unstrained tissue equivalents. CONCLUSIONS: The cell and fibre alignment of the engineered PDL was precisely guided by mechanical shear stress along the direction of principal strain vector using a custom-designed bioreactor, suggesting that the enhanced functional property of engineered PDL constructs could be achieved with this technique.
Authors: Juan Wang; Joseph Koelbl; Anuraag Boddupalli; Zhiqi Yao; Kaitlin M Bratlie; Ian C Schneider Journal: Integr Biol (Camb) Date: 2018-11-12 Impact factor: 2.192
Authors: Sophia P Pilipchuk; Alberto Monje; Yizu Jiao; Jie Hao; Laura Kruger; Colleen L Flanagan; Scott J Hollister; William V Giannobile Journal: Adv Healthc Mater Date: 2016-01-28 Impact factor: 9.933