Literature DB >> 22082691

Electron tomography of cells.

Lu Gan1, Grant J Jensen.   

Abstract

The electron microscope has contributed deep insights into biological structure since its invention nearly 80 years ago. Advances in instrumentation and methodology in recent decades have now enabled electron tomography to become the highest resolution three-dimensional (3D) imaging technique available for unique objects such as cells. Cells can be imaged either plastic-embedded or frozen-hydrated. Then the series of projection images are aligned and back-projected to generate a 3D reconstruction or 'tomogram'. Here, we review how electron tomography has begun to reveal the molecular organization of cells and how the existing and upcoming technologies promise even greater insights into structural cell biology.

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Year:  2011        PMID： 22082691     DOI： 10.1017/S0033583511000102

Source DB:  PubMed          Journal:  Q Rev Biophys        ISSN： 0033-5835            Impact factor:   5.318


  56 in total

1.  Electron cryotomography studies of maturing HIV-1 particles reveal the assembly pathway of the viral core.

Authors:  Cora L Woodward; Sarah N Cheng; Grant J Jensen
Journal:  J Virol       Date:  2014-11-12       Impact factor: 5.103

Review 2.  Out with the old and in with the new: rapid specimen preparation procedures for electron microscopy of sectioned biological material.

Authors:  Kent L McDonald
Journal:  Protoplasma       Date:  2013-11-21       Impact factor: 3.356

3.  The Caltech Tomography Database and Automatic Processing Pipeline.

Authors:  H Jane Ding; Catherine M Oikonomou; Grant J Jensen
Journal:  J Struct Biol       Date:  2015-06-15       Impact factor: 2.867

4.  In situ localization of N and C termini of subunits of the flagellar nexin-dynein regulatory complex (N-DRC) using SNAP tag and cryo-electron tomography.

Authors:  Kangkang Song; Junya Awata; Douglas Tritschler; Raqual Bower; George B Witman; Mary E Porter; Daniela Nicastro
Journal:  J Biol Chem       Date:  2015-01-06       Impact factor: 5.157

5.  Imaging bacterial 3D motion using digital in-line holographic microscopy and correlation-based de-noising algorithm.

Authors:  Mehdi Molaei; Jian Sheng
Journal:  Opt Express       Date:  2014-12-29       Impact factor: 3.894

6.  In situ imaging of the bacterial flagellar motor disassembly and assembly processes.

Authors:  Mohammed Kaplan; Poorna Subramanian; Debnath Ghosal; Catherine M Oikonomou; Sahand Pirbadian; Ruth Starwalt-Lee; Shrawan Kumar Mageswaran; Davi R Ortega; Jeffrey A Gralnick; Mohamed Y El-Naggar; Grant J Jensen
Journal:  EMBO J       Date:  2019-05-20       Impact factor: 11.598

7.  Preparation and Observation of Thick Biological Samples by Scanning Transmission Electron Tomography.

Authors:  Sylvain Trépout; Philippe Bastin; Sergio Marco
Journal:  J Vis Exp       Date:  2017-03-12       Impact factor: 1.355

8.  High Rac1 activity is functionally translated into cytosolic structures with unique nanoscale cytoskeletal architecture.

Authors:  Daniel J Marston; Karen L Anderson; Mark F Swift; Marie Rougie; Christopher Page; Klaus M Hahn; Niels Volkmann; Dorit Hanein
Journal:  Proc Natl Acad Sci U S A       Date:  2019-01-10       Impact factor: 11.205

9.  Chemotaxis cluster 1 proteins form cytoplasmic arrays in Vibrio cholerae and are stabilized by a double signaling domain receptor DosM.

Authors:  Ariane Briegel; Davi R Ortega; Petra Mann; Andreas Kjær; Simon Ringgaard; Grant J Jensen
Journal:  Proc Natl Acad Sci U S A       Date:  2016-08-29       Impact factor: 11.205

10.  Unclosed HIV-1 capsids suggest a curled sheet model of assembly.

Authors:  Zhiheng Yu; Megan J Dobro; Cora L Woodward; Artem Levandovsky; Cindy M Danielson; Virginie Sandrin; Jiong Shi; Christopher Aiken; Roya Zandi; Thomas J Hope; Grant J Jensen
Journal:  J Mol Biol       Date:  2012-10-16       Impact factor: 5.469
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