Literature DB >> 22081561

Bacterial whole-cell biosensor for glutamine with applications for quantifying and visualizing glutamine in plants.

Michael J Tessaro1, Sameh S M Soliman, Manish N Raizada.   

Abstract

A whole-cell biosensor for glutamine (GlnLux) was constructed by transforming an Escherichia coli glutamine (Gln) auxotroph with a constitutive lux reporter gene. Measurements of Gln in plant extracts using GlnLux correlated with quantification using high-performance liquid chromatography (Spearman's r = 0.95). GlnLux permitted charge-coupled-device (CCD) imaging of Gln from whole plant organs.

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Year:  2011        PMID： 22081561      PMCID： PMC3255742          DOI： 10.1128/AEM.05261-11

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  18 in total

1.  Biological sensor for sucrose availability: relative sensitivities of various reporter genes.

Authors:  W G Miller; M T Brandl; B Quiñones; S E Lindow
Journal:  Appl Environ Microbiol       Date:  2001-03       Impact factor: 4.792

2.  Multiple repetitive elements and organization of the lux operons of luminescent terrestrial bacteria.

Authors:  E A Meighen; R B Szittner
Journal:  J Bacteriol       Date:  1992-08       Impact factor: 3.490

3.  A biological sensor for iron available to bacteria in their habitats on plant surfaces.

Authors:  J E Loper; S E Lindow
Journal:  Appl Environ Microbiol       Date:  1994-06       Impact factor: 4.792

4.  A bioluminescent Escherichia coli auxotroph for use in an in vitro lysine availability assay.

Authors:  A M Erickson; I B Díaz; Y M Kwon; S C Ricke
Journal:  J Microbiol Methods       Date:  2000-05       Impact factor: 2.363

Review 5.  The role of glutamine synthetase and glutamate dehydrogenase in nitrogen assimilation and possibilities for improvement in the nitrogen utilization of crops.

Authors:  Ben J Miflin; Dimah Z Habash
Journal:  J Exp Bot       Date:  2002-04       Impact factor: 6.992

6.  Mapping of sugar and amino acid availability in soil around roots with bacterial sensors of sucrose and tryptophan

Authors: 
Journal:  Appl Environ Microbiol       Date:  1999-06       Impact factor: 4.792

7.  Structural and metabolic transitions of C4 leaf development and differentiation defined by microscopy and quantitative proteomics in maize.

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Journal:  Plant Cell       Date:  2010-11-16       Impact factor: 11.277

8.  Analysis of glutamine, glutamate, pyroglutamate, and GABA in cerebrospinal fluid using ion pairing HPLC with positive electrospray LC/MS/MS.

Authors:  James A Eckstein; Gina M Ammerman; Jessica M Reveles; Bradley L Ackermann
Journal:  J Neurosci Methods       Date:  2008-03-18       Impact factor: 2.390

9.  Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection.

Authors:  Tomoya Baba; Takeshi Ara; Miki Hasegawa; Yuki Takai; Yoshiko Okumura; Miki Baba; Kirill A Datsenko; Masaru Tomita; Barry L Wanner; Hirotada Mori
Journal:  Mol Syst Biol       Date:  2006-02-21       Impact factor: 11.429

10.  A rapid biosensor-based method for quantification of free and glucose-conjugated salicylic acid.

Authors:  Christopher T Defraia; Eric A Schmelz; Zhonglin Mou
Journal:  Plant Methods       Date:  2008-12-31       Impact factor: 4.993
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  4 in total

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Journal:  PLoS One       Date:  2012-07-19       Impact factor: 3.240

2.  Biosensor-based spatial and developmental mapping of maize leaf glutamine at vein-level resolution in response to different nitrogen rates and uptake/assimilation durations.

Authors:  Travis L Goron; Manish N Raizada
Journal:  BMC Plant Biol       Date:  2016-10-21       Impact factor: 4.215

3.  Whole-cell Escherichia coli lactate biosensor for monitoring mammalian cell cultures during biopharmaceutical production.

Authors:  Lisa Goers; Catherine Ainsworth; Cher Hui Goey; Cleo Kontoravdi; Paul S Freemont; Karen M Polizzi
Journal:  Biotechnol Bioeng       Date:  2017-02-23       Impact factor: 4.530

4.  A Biosensor-Based Leaf Punch Assay for Glutamine Correlates to Symbiotic Nitrogen Fixation Measurements in Legumes to Permit Rapid Screening of Rhizobia Inoculants under Controlled Conditions.

Authors:  Malinda S Thilakarathna; Nicholas Moroz; Manish N Raizada
Journal:  Front Plant Sci       Date:  2017-10-09       Impact factor: 5.753
  4 in total

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