PURPOSE: To assess the efficacy of dodecafluoropentane emulsion (DDFPe), a nanodroplet emulsion with significant oxygen transport potential, in decreasing infarct volume in an insoluble-emboli rabbit stroke model. MATERIALS AND METHODS: New Zealand White rabbits (N = 64; weight, 5.1 ± 0.50 kg) underwent angiography and received embolic spheres in occluded internal carotid artery branches. Rabbits were randomly assigned to groups in 4-hour and 7-hour studies. Four-hour groups included control (n = 7, embolized without treatment) and DDFPe treatment 30 minutes before stroke (n = 7), at stroke onset (n = 8), and 30 minutes (n = 5), 1 hour (n = 7), 2 hours (n = 5), or 3 hours after stroke (n = 6). Seven-hour groups included control (n = 6) and DDFPe at 1 hour (n = 8) and 6 hours after stroke (n = 5). DDFPe dose was a 2% weight/volume intravenous injection (0.6 mL/kg) repeated every 90 minutes as time allowed. After euthanasia, infarct volume was determined by vital stains on brain sections. RESULTS: At 4 hours, median infarct volume decreased for all DDFPe treatment times (pretreatment, 0.30% [P = .004]; onset, 0.20% [P = .004]; 30 min, 0.35% [P = .009]; 1 h, 0.30% [P = .01]; 2 h, 0.40% [P = .009]; and 3 h, 0.25% [P = .003]) compared with controls (3.20%). At 7 hours, median infarct volume decreased with treatment at 1 hour (0.25%; P = .007) but not at 6 hours (1.4%; P = .49) compared with controls (2.2%). CONCLUSIONS: Intravenous DDFPe in an animal model decreases infarct volumes and protects brain tissue from ischemia, justifying further investigation.
PURPOSE: To assess the efficacy of dodecafluoropentane emulsion (DDFPe), a nanodroplet emulsion with significant oxygen transport potential, in decreasing infarct volume in an insoluble-emboli rabbit stroke model. MATERIALS AND METHODS: New Zealand White rabbits (N = 64; weight, 5.1 ± 0.50 kg) underwent angiography and received embolic spheres in occluded internal carotid artery branches. Rabbits were randomly assigned to groups in 4-hour and 7-hour studies. Four-hour groups included control (n = 7, embolized without treatment) and DDFPe treatment 30 minutes before stroke (n = 7), at stroke onset (n = 8), and 30 minutes (n = 5), 1 hour (n = 7), 2 hours (n = 5), or 3 hours after stroke (n = 6). Seven-hour groups included control (n = 6) and DDFPe at 1 hour (n = 8) and 6 hours after stroke (n = 5). DDFPe dose was a 2% weight/volume intravenous injection (0.6 mL/kg) repeated every 90 minutes as time allowed. After euthanasia, infarct volume was determined by vital stains on brain sections. RESULTS: At 4 hours, median infarct volume decreased for all DDFPe treatment times (pretreatment, 0.30% [P = .004]; onset, 0.20% [P = .004]; 30 min, 0.35% [P = .009]; 1 h, 0.30% [P = .01]; 2 h, 0.40% [P = .009]; and 3 h, 0.25% [P = .003]) compared with controls (3.20%). At 7 hours, median infarct volume decreased with treatment at 1 hour (0.25%; P = .007) but not at 6 hours (1.4%; P = .49) compared with controls (2.2%). CONCLUSIONS: Intravenous DDFPe in an animal model decreases infarct volumes and protects brain tissue from ischemia, justifying further investigation.
Authors: Hans-Christoph Diener; Kennedy R Lees; Patrick Lyden; Jim Grotta; Antoni Davalos; Stephen M Davis; Ashfaq Shuaib; Tim Ashwood; Warren Wasiewski; Vivian Alderfer; Hans-Goran Hårdemark; Larry Rodichok Journal: Stroke Date: 2008-03-27 Impact factor: 7.914
Authors: Zhonglin Liu; Christy Barber; Akash Gupta; Li Wan; Young-Wook Won; Lars R Furenlid; Qin Chen; Ankit A Desai; Ming Zhao; David A Bull; Evan C Unger; Diego R Martin Journal: Nucl Med Biol Date: 2019-01-17 Impact factor: 2.408
Authors: William C Culp; Sean D Woods; Aliza T Brown; John D Lowery; Leah J Hennings; Robert D Skinner; Michael J Borrelli; Paula K Roberson Journal: J Neurosci Methods Date: 2012-11-08 Impact factor: 2.390
Authors: Christine Arthur; Lin Song; William Culp; Aliza Brown; Michael Borrelli; Robert Skinner; Howard Hendrickson Journal: AAPS J Date: 2016-12-27 Impact factor: 4.009
Authors: M Christine Arthur; Aliza Brown; Kristen Carlson; John Lowery; Robert D Skinner; William C Culp Journal: Mol Neurobiol Date: 2016-08-08 Impact factor: 5.590
Authors: S D Woods; R D Skinner; A M Ricca; A T Brown; J D Lowery; M J Borrelli; J O Lay; W C Culp Journal: Mol Neurobiol Date: 2013-06-28 Impact factor: 5.590
Authors: Aliza Brown; Sean Woods; Robert Skinner; Jeff Hatton; John Lowery; Paula Roberson; Leah Hennings; William C Culp Journal: Open Neurol J Date: 2013-10-31