OBJECTIVE: To investigate the effect of androgens on the expression of genes involved in oxidative stress resistance in decidualized human endometrial stromal cells (HESCs). DESIGN: In vitro experiment. SETTING: University hospital. PATIENT(S): Premenopausal women undergoing hysterectomy for uterine fibroids. INTERVENTION(S): Human endometrial stromal cells isolated from hysterectomy specimens were decidualized with 8-bromo-cyclic adenosine monophosphate (8-br-cAMP) and P in the presence or absence of dihydrotestosterone (DHT) at various concentrations. Hydrogen peroxide was used as a source of reactive oxygen species. MAIN OUTCOME MEASURE(S): Prolactin secretion, apoptosis, FOXO1, and the free radical scavengers superoxide dismutase 2 (SOD2) and SOD1 protein expression. RESULT(S): Prolactin production was induced in HESCs in response to 8-br-cAMP and P. Dihydrotestosterone further enhanced the secretion of PRL in cells treated with 8-br-cAMP plus P. The effect of DHT was blocked by the antiandrogen flutamide. Dihydrotestosterone enhanced resistance to oxidative stress-induced apoptosis on decidualized HESCs. Moreover, DHT enhanced FOXO1 expression in parallel with increased SOD2 protein but not with SOD1. CONCLUSION(S): Androgens might play a critical role in the decidualization process at the time of embryo implantation and trophoblast invasion by promoting resistance to oxidative stress.
OBJECTIVE: To investigate the effect of androgens on the expression of genes involved in oxidative stress resistance in decidualized human endometrial stromal cells (HESCs). DESIGN: In vitro experiment. SETTING: University hospital. PATIENT(S): Premenopausal women undergoing hysterectomy for uterine fibroids. INTERVENTION(S): Human endometrial stromal cells isolated from hysterectomy specimens were decidualized with 8-bromo-cyclic adenosine monophosphate (8-br-cAMP) and P in the presence or absence of dihydrotestosterone (DHT) at various concentrations. Hydrogen peroxide was used as a source of reactive oxygen species. MAIN OUTCOME MEASURE(S): Prolactin secretion, apoptosis, FOXO1, and the free radical scavengers superoxide dismutase 2 (SOD2) and SOD1 protein expression. RESULT(S): Prolactin production was induced in HESCs in response to 8-br-cAMP and P. Dihydrotestosterone further enhanced the secretion of PRL in cells treated with 8-br-cAMP plus P. The effect of DHT was blocked by the antiandrogen flutamide. Dihydrotestosterone enhanced resistance to oxidative stress-induced apoptosis on decidualized HESCs. Moreover, DHT enhanced FOXO1 expression in parallel with increased SOD2 protein but not with SOD1. CONCLUSION(S): Androgens might play a critical role in the decidualization process at the time of embryo implantation and trophoblast invasion by promoting resistance to oxidative stress.
Authors: Laura Detti; Rebecca A Uhlmann; Nicole M Fletcher; Michael P Diamond; Ghassan M Saed Journal: Fertil Steril Date: 2013-06-24 Impact factor: 7.329
Authors: Cristina Antinozzi; Guglielmo Duranti; Roberta Ceci; Marco Lista; Stefania Sabatini; Daniela Caporossi; Luigi Di Luigi; Paolo Sgrò; Ivan Dimauro Journal: Int J Mol Sci Date: 2022-06-12 Impact factor: 6.208
Authors: Douglas A Gibson; Ioannis Simitsidellis; Fiona L Cousins; Hilary O D Critchley; Philippa T K Saunders Journal: Sci Rep Date: 2016-01-28 Impact factor: 4.379