| Literature DB >> 22070407 |
Hirokazu Nagaoka, Yasunobu Sato, Xiaomao Xie, Hideyuki Hata, Masaru Eguchi, Nobuki Sakurai, Takeshi Watanabe, Hiroshi Saitoh, Akihiko Kondo, Satoru Sugita, Noriyuki Ohnishi.
Abstract
Because current homogeneous immunoassays show some limitations, particularly low sensitivity, we developed a new immunoassay to overcome these limitations. The approach was based on magnetic nanoparticles with a thermoresponsive polymer layer, a negatively charged polymer, and streptavidin-biotin-based antibody-antigen detection and yielded higher sensitivity than commonly used heterogeneous immunoassays. Because no special equipment is needed, it can be applied to currently available absorbance-based systems for high-throughput assays.Entities:
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Year: 2011 PMID： 22070407 DOI： 10.1021/ac201814n
Source DB: PubMed Journal: Anal Chem ISSN： 0003-2700 Impact factor: 6.986