PURPOSE: The key anticancer agent, CPT-11 (irinotecan hydrochloride), induces severe diarrhea clinically. We investigated the effect of a Kampo medicine, Dai-kenchu-to (DKT), on CPT-11-induced intestinal injuries in rats. METHODS: Twenty-four male Wistar rats were divided into three groups: a control group; a CPT-11 group, given CPT-11 150 mg/kg intraperitoneally for 2 days; and a DKT group, given DKT 300 mg/kg orally for 5 days with CPT-11 150 mg/kg intraperitoneally on days 4 and 5. The rats were killed on day 6. RESULTS: Interleukin (IL)-1β, IL-12, interferon (IFN)-γ, and tumor necrosis factor-α expression in the small intestine of the CPT-11 group was significantly higher than that of the control group. Interleukin-1β and IFN-γ expression was improved significantly by DKT (P < 0.05). The number and height of jejuna villi, injury score, and apoptosis index in the CPT-11 group were improved significantly by DKT (P < 0.05). CONCLUSIONS: DKT suppressed CPT-11 induced inflammatory cytokines and apoptosis in the intestinal mucosa and maintained the mucosal integrity.
PURPOSE: The key anticancer agent, CPT-11 (irinotecan hydrochloride), induces severe diarrhea clinically. We investigated the effect of a Kampo medicine, Dai-kenchu-to (DKT), on CPT-11-induced intestinal injuries in rats. METHODS: Twenty-four male Wistar rats were divided into three groups: a control group; a CPT-11 group, given CPT-11 150 mg/kg intraperitoneally for 2 days; and a DKT group, given DKT 300 mg/kg orally for 5 days with CPT-11 150 mg/kg intraperitoneally on days 4 and 5. The rats were killed on day 6. RESULTS: Interleukin (IL)-1β, IL-12, interferon (IFN)-γ, and tumor necrosis factor-α expression in the small intestine of the CPT-11 group was significantly higher than that of the control group. Interleukin-1β and IFN-γ expression was improved significantly by DKT (P < 0.05). The number and height of jejuna villi, injury score, and apoptosis index in the CPT-11 group were improved significantly by DKT (P < 0.05). CONCLUSIONS: DKT suppressed CPT-11 induced inflammatory cytokines and apoptosis in the intestinal mucosa and maintained the mucosal integrity.
Authors: R Ohno; K Okada; T Masaoka; A Kuramoto; T Arima; Y Yoshida; H Ariyoshi; M Ichimaru; Y Sakai; M Oguro Journal: J Clin Oncol Date: 1990-11 Impact factor: 44.544
Authors: Serdar H Iskit; Halil Tugtepe; Suat H Ayyildiz; Esin Kotiloglu; Tolga E Dagli; Berrak C Yeğen Journal: Pediatr Surg Int Date: 2005-05-13 Impact factor: 1.827
Authors: Edith A Perez; David W Hillman; James A Mailliard; James N Ingle; J Michael Ryan; Tom R Fitch; Kendrith M Rowland; Carl G Kardinal; James E Krook; John W Kugler; Shaker R Dakhil Journal: J Clin Oncol Date: 2004-07-15 Impact factor: 44.544
Authors: Rachel J Gibson; Joanne M Bowen; Mark R B Inglis; Adrian G Cummins; Dorothy M K Keefe Journal: J Gastroenterol Hepatol Date: 2003-09 Impact factor: 4.029
Authors: Richard M Logan; Andrea M Stringer; Joanne M Bowen; Ann S-J Yeoh; Rachel J Gibson; Stephen T Sonis; Dorothy M K Keefe Journal: Cancer Treat Rev Date: 2007-05-15 Impact factor: 12.111
Authors: Chao Deng; Bo Deng; Liqun Jia; Huangying Tan; Pan Zhang; Sida Liu; Yanan Zhang; Aiping Song; Lin Pan Journal: Evid Based Complement Alternat Med Date: 2017-01-12 Impact factor: 2.629