Literature DB >> 22054546

Purification of BAC DNA for high-efficiency transgenesis.

Rajendra K Gangalum1, Zhe Jing, Yoshiko Nagaoka, Meisheng Jiang, Suraj P Bhat, Suraj P Bhat.   

Abstract

An unresolved bottleneck in bacterial artificial chromosome (BAC) transgenesis is low efficiency generation of founder mice because of suboptimal quality of the manipulated BAC DNA. Using mini-gel electrophoresis and electro-elution that circumvents CsCl(2) centrifugation, column chromatography, and resin purifications, we have used RECOCHIP, a commercially available dialysis cassette for the purification of BAC DNA that generates transgenic founders with up to 80% efficiency.

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Year:  2011        PMID： 22054546     DOI： 10.2144/000113763

Source DB:  PubMed          Journal:  Biotechniques        ISSN： 0736-6205            Impact factor:   1.993


  6 in total

1.  Expression of the HSF4 DNA binding domain-EGFP hybrid gene recreates early childhood lamellar cataract in transgenic mice.

Authors:  Rajendra K Gangalum; Zhe Jing; Ankur M Bhat; Josh Lee; Yoshiko Nagaoka; Sophie X Deng; Meisheng Jiang; Suraj P Bhat
Journal:  Invest Ophthalmol Vis Sci       Date:  2014-08-28       Impact factor: 4.799

2.  HSF4 mutation p.Arg116His found in age-related cataracts and in normal populations produces childhood lamellar cataract in transgenic mice.

Authors:  Zhe Jing; Rajendra K Gangalum; Ankur M Bhat; Yoshiko Nagaoka; Meisheng Jiang; Suraj P Bhat
Journal:  Hum Mutat       Date:  2014-07-23       Impact factor: 4.878

3.  Bioluminescence imaging of β cells and intrahepatic insulin gene activity under normal and pathological conditions.

Authors:  Tokio Katsumata; Hisashi Oishi; Yukari Sekiguchi; Haruka Nagasaki; Dhouha Daassi; Pei-Han Tai; Masatsugu Ema; Takashi Kudo; Satoru Takahashi
Journal:  PLoS One       Date:  2013-04-04       Impact factor: 3.240

4.  A gene-specific non-enhancer sequence is critical for expression from the promoter of the small heat shock protein gene αB-crystallin.

Authors:  Zhe Jing; Rajendra K Gangalum; Dennis C Mock; Suraj P Bhat
Journal:  Hum Genomics       Date:  2014-03-03       Impact factor: 4.639

5.  Assembly of eukaryotic algal chromosomes in yeast.

Authors:  Bogumil J Karas; Bhuvan Molparia; Jelena Jablanovic; Wolfgang J Hermann; Ying-Chi Lin; Christopher L Dupont; Christian Tagwerker; Isaac T Yonemoto; Vladimir N Noskov; Ray-Yuan Chuang; Andrew E Allen; John I Glass; Clyde A Hutchison; Hamilton O Smith; J Craig Venter; Philip D Weyman
Journal:  J Biol Eng       Date:  2013-12-10       Impact factor: 4.355

6.  Generation and characterization of Ins1-cre-driver C57BL/6N for exclusive pancreatic beta cell-specific Cre-loxP recombination.

Authors:  Yoshikazu Hasegawa; Yoko Daitoku; Seiya Mizuno; Yoko Tanimoto; Saori Mizuno-Iijima; Miki Matsuo; Noriko Kajiwara; Masatsugu Ema; Hisashi Oishi; Yoshihiro Miwa; Kazuyuki Mekada; Atsushi Yoshiki; Satoru Takahashi; Fumihiro Sugiyama; Ken-ichi Yagami
Journal:  Exp Anim       Date:  2014
  6 in total

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