Literature DB >> 2204554

Sperm bound to zona pellucida in hemizona assay demonstrate acrosome reaction when stained with T-6 antibody.

C C Coddington1, D L Fulgham, N J Alexander, D Johnson, J C Herr, G D Hodgen.   

Abstract

A monoclonal antibody, T-6, useful for detecting acrosome-reacted sperm based on an immunofluorescent assay, was employed to evaluate acrosomal status of human sperm that were tightly bound to hemisected human zonae pellucidae (hemizona assay). Over 90% of the bound sperm evaluated exhibited immunofluorescent patterns indicative of acrosome reaction. This staining method for evaluating the acrosomal status of sperm bound to the zona pellucida may enable definition of a group of male infertility patients heretofore not recognized.

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Year:  1990        PMID： 2204554

Source DB:  PubMed          Journal:  Fertil Steril        ISSN： 0015-0282            Impact factor:   7.329


  5 in total

1.  Physiological induction of the acrosome reaction in human sperm: validation of a microassay using minimal volumes of solubilized, homologous zona pellucida.

Authors:  D R Franken; H S Bastiaan; S C Oehninger
Journal:  J Assist Reprod Genet       Date:  2000-08       Impact factor: 3.412

2.  Physiological induction of the acrosome reaction in human sperm: validation of a microassay using minimal volumes of solubilized, homologous zona pellucida.

Authors:  D R Franken; H S Bastiaan; S C Oehninger
Journal:  J Assist Reprod Genet       Date:  2000-03       Impact factor: 3.412

3.  Use of failed-fertilized oocytes for diagnostic zona binding purposes after sperm binding improvement with a modified medium.

Authors:  R Henkel; C Müller; T Stalf; W B Schill; D R Franken
Journal:  J Assist Reprod Genet       Date:  1999-01       Impact factor: 3.412

4.  Hemizona assay: use of fresh versus salt-stored human oocytes to evaluate sperm binding potential to the zona pellucida.

Authors:  T F Kruger; S Oehninger; D R Franken; G D Hodgen
Journal:  J In Vitro Fert Embryo Transf       Date:  1991-06

5.  Validation of the hemizona assay (HZA) in a monkey model. II. Kinetics of binding and influence of cryopreserved-thawed spermatozoa.

Authors:  S Oehninger; M Morshedi; H Ertunc; C Philput; S M Bocca; A A Acosta; G D Hodgen
Journal:  J Assist Reprod Genet       Date:  1993-05       Impact factor: 3.412
  5 in total

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