High-resolution cantilever biosensor resonating at air-liquid in a microchannel.

We have developed a highly mass-sensitive cantilever resonating at the interface of air and liquid. The cantilever is applicable as a biosensor by measuring its resonance frequency shift associated with the selective trapping of target molecules. One surface of the cantilever facing to the liquid is functionalized for label-free detection, while the opposite side is exposed to air to improve the resonance characteristics, such as the quality factor. The quality factor at resonance is 15, which is 50% higher than the same cantilever in liquid. The beam was excited through the photothermal effect of a power modulated laser and detected by laser Doppler velocimetry. Due to the proposed configuration, the signal-to-noise-ratio is 5.7 times larger than the completely submerged case. A micro-slit around the cantilever separates the air and liquid phases at a meniscus. We analyzed the cantilever motion including the meniscus membrane, and examined the effect of surface tension by applying various solutions. A slit width of 6 μm was found to give the best performance within the few prototypes. We measured the covalent immobilization of antibody molecules on a cantilever surface for three different concentrations: 20, 40, and 80 μg ml(-1). The kinetics measured by both resonance frequency shift of the cantilever and fluorescent intensity showed good agreement.