BACKGROUND: Volatile organic compounds (VOCs) present in breath of lung cancer patients and released by lung cancer cells were determined. As a reference sample breath collected from healthy volunteers and lung segments of cells with proper morphology were used. METHODS: Solid phase microextraction and gas chromato-graphy mass spectrometry (SPME-GC/MS) method was applied for VOC analysis. Statistical methods have been developed to find similarities between patterns of investigated compounds within three populations of samples: exhaled air samples from patients with lung cancer, headspace of lung tissues with healthy and cancer cells. RESULTS: Quantitative analysis of VOCs in the headspace of lung tissues revealed that cancer cells released higher concentrations of ethanol, acetone, carbon disulfide, dimethyl sulfide, 1-propanol, 2-propanol, 2-butanone and 2-pentanone than healthy tissues. The increase of concentration of the same compounds was observed in the breath of patients with lung cancer in comparison to breath from healthy non-smoking volunteers. CONCLUSIONS: Detection of lung cancer is possible by volatile biomarkers analysis in breath.
BACKGROUND: Volatile organic compounds (VOCs) present in breath of lung cancerpatients and released by lung cancer cells were determined. As a reference sample breath collected from healthy volunteers and lung segments of cells with proper morphology were used. METHODS: Solid phase microextraction and gas chromato-graphy mass spectrometry (SPME-GC/MS) method was applied for VOC analysis. Statistical methods have been developed to find similarities between patterns of investigated compounds within three populations of samples: exhaled air samples from patients with lung cancer, headspace of lung tissues with healthy and cancer cells. RESULTS: Quantitative analysis of VOCs in the headspace of lung tissues revealed that cancer cells released higher concentrations of ethanol, acetone, carbon disulfide, dimethyl sulfide, 1-propanol, 2-propanol, 2-butanone and 2-pentanone than healthy tissues. The increase of concentration of the same compounds was observed in the breath of patients with lung cancer in comparison to breath from healthy non-smoking volunteers. CONCLUSIONS: Detection of lung cancer is possible by volatile biomarkers analysis in breath.
Authors: Ehab I Mohamed; Marwa A Mohamed; Samir M Abdel-Mageed; Taher S Abdel-Mohdy; Mohamed I Badawi; Samy H Darwish Journal: J Appl Biomed Date: 2019-01-10 Impact factor: 1.797
Authors: Arjan Pol; G Herma Renkema; Albert Tangerman; Edwin G Winkel; Udo F Engelke; Arjan P M de Brouwer; Kent C Lloyd; Renee S Araiza; Lambert van den Heuvel; Heymut Omran; Heike Olbrich; Marijn Oude Elberink; Christian Gilissen; Richard J Rodenburg; Jörn Oliver Sass; K Otfried Schwab; Hendrik Schäfer; Hanka Venselaar; J Silvia Sequeira; Huub J M Op den Camp; Ron A Wevers Journal: Nat Genet Date: 2017-12-18 Impact factor: 38.330