BACKGROUND: The mucosal layer of the colon contains metabolism-related fluorophores, such as reduced nicotinamide adenine dinucleotide (NADH), which might have the potential to serve as biomarkers for detecting neoplasia. OBJECTIVE: To examine NADH fluorescence in human colonic adenoma while eliminating the effect of hemoglobin absorption and to develop a novel imaging technique for precise detection of adenomas. DESIGN: Cross-sectional study. PATIENTS AND INTERVENTIONS: A total of 66 endoscopically resected colonic polyps were investigated. After serial acquisition of autofluorescence images between 450 and 490 nm illuminated with dual-wavelength excitation at 365 nm (F(365ex)) and 405 nm (F(405ex)) on cross sections of the samples, ratio images were created by dividing F(365ex) by F(405ex). The excitation-emission wavelength combinations in F(365ex) and F(405ex) were optimized for NADH fluorescence and reference fluorescence. MAIN OUTCOME MEASUREMENTS: The F(365ex)/F(405ex) ratio in the tumorous (T) and normal (N) mucosa. RESULTS: F(365ex)/F(405ex) ratio images showed a 1.81- and 1.12-fold higher signal intensity in the adenomas and hyperplastic polyps, respectively, than in the adjacent normal mucosa. The ratio between signal intensities in tumorous mucosa and normal mucosa in F(365ex)/F(405ex) ratio images for tubular adenomas was significantly higher than that for hyperplastic polyps. The signal intensity in F(365ex)/F(405ex) ratio images was not correlated with the hemoglobin concentration index evaluated by reflection images at 550 nm and 610 nm. Diminutive adenomas (<5 mm) and large adenomas were well discriminated in F(365ex)/F(405ex) ratio images. LIMITATIONS: Ex vivo experiment. CONCLUSIONS: These results suggest that the precise measurement of NADH fluorescence intensity together with eliminating the influence of blood hemoglobin concentration serves as a method for visualizing colonic adenomas and that the dual-wavelength excitation method is a promising technique applicable to endoscopic detection of early colonic adenomas.
BACKGROUND: The mucosal layer of the colon contains metabolism-related fluorophores, such as reduced nicotinamide adenine dinucleotide (NADH), which might have the potential to serve as biomarkers for detecting neoplasia. OBJECTIVE: To examine NADH fluorescence in humancolonic adenoma while eliminating the effect of hemoglobin absorption and to develop a novel imaging technique for precise detection of adenomas. DESIGN: Cross-sectional study. PATIENTS AND INTERVENTIONS: A total of 66 endoscopically resected colonic polyps were investigated. After serial acquisition of autofluorescence images between 450 and 490 nm illuminated with dual-wavelength excitation at 365 nm (F(365ex)) and 405 nm (F(405ex)) on cross sections of the samples, ratio images were created by dividing F(365ex) by F(405ex). The excitation-emission wavelength combinations in F(365ex) and F(405ex) were optimized for NADH fluorescence and reference fluorescence. MAIN OUTCOME MEASUREMENTS: The F(365ex)/F(405ex) ratio in the tumorous (T) and normal (N) mucosa. RESULTS: F(365ex)/F(405ex) ratio images showed a 1.81- and 1.12-fold higher signal intensity in the adenomas and hyperplastic polyps, respectively, than in the adjacent normal mucosa. The ratio between signal intensities in tumorous mucosa and normal mucosa in F(365ex)/F(405ex) ratio images for tubular adenomas was significantly higher than that for hyperplastic polyps. The signal intensity in F(365ex)/F(405ex) ratio images was not correlated with the hemoglobin concentration index evaluated by reflection images at 550 nm and 610 nm. Diminutive adenomas (<5 mm) and large adenomas were well discriminated in F(365ex)/F(405ex) ratio images. LIMITATIONS: Ex vivo experiment. CONCLUSIONS: These results suggest that the precise measurement of NADH fluorescence intensity together with eliminating the influence of blood hemoglobin concentration serves as a method for visualizing colonic adenomas and that the dual-wavelength excitation method is a promising technique applicable to endoscopic detection of early colonic adenomas.
Authors: Silas J Leavesley; Mikayla Walters; Carmen Lopez; Thomas Baker; Peter F Favreau; Thomas C Rich; Paul F Rider; Carole W Boudreaux Journal: J Biomed Opt Date: 2016-10-01 Impact factor: 3.170
Authors: Jacobus Burggraaf; Ingrid M C Kamerling; Paul B Gordon; Lenneke Schrier; Marieke L de Kam; Andrea J Kales; Ragnar Bendiksen; Bård Indrevoll; Roger M Bjerke; Siver A Moestue; Siavash Yazdanfar; Alexandra M J Langers; Marit Swaerd-Nordmo; Geir Torheim; Madhuri V Warren; Hans Morreau; Philip W Voorneveld; Tessa Buckle; Fijs W B van Leeuwen; Liv-Ingrid Ødegårdstuen; Grethe T Dalsgaard; Andrew Healey; James C H Hardwick Journal: Nat Med Date: 2015-07-13 Impact factor: 53.440
Authors: Stephan Rogalla; Krzysztof Flisikowski; Dimitris Gorpas; Aaron T Mayer; Tatiana Flisikowska; Michael J Mandella; Xiaopeng Ma; Kerriann M Casey; Stephen A Felt; Dieter Saur; Vasilis Ntziachristos; Angelika Schnieke; Christopher H Contag; Sanjiv S Gambhir; Stefan Harmsen Journal: Adv Funct Mater Date: 2019-10-10 Impact factor: 18.808