BACKGROUND: An Italian interlaboratory study was run in 2010 to assess the performance of Blood Transfusion Services in detecting the genome of West Nile virus (WNV) in plasma. MATERIALS AND METHODS: Each laboratory received a panel of samples containing four samples negative for WNV and six positive samples with a nominal viral concentration close to or below the 95% detection limit of two commercially available nucleic acid amplification tests (NAT) for WNV, the PROCLEIX® WNV kit and the Cobas® TaqScreen West Nile Virus kit. RESULTS: Ten laboratories took part in the study. All correctly identified the positive samples with a viral concentration above the 95% detection limit. No pre- or post-analytical errors were observed. CONCLUSIONS: The interlaboratory study run in 2010 allowed participants to assess the performance of the NAT methods applied in their seasonal routine screening of blood donations.
BACKGROUND: An Italian interlaboratory study was run in 2010 to assess the performance of Blood Transfusion Services in detecting the genome of West Nile virus (WNV) in plasma. MATERIALS AND METHODS: Each laboratory received a panel of samples containing four samples negative for WNV and six positive samples with a nominal viral concentration close to or below the 95% detection limit of two commercially available nucleic acid amplification tests (NAT) for WNV, the PROCLEIX® WNV kit and the Cobas® TaqScreen West Nile Virus kit. RESULTS: Ten laboratories took part in the study. All correctly identified the positive samples with a viral concentration above the 95% detection limit. No pre- or post-analytical errors were observed. CONCLUSIONS: The interlaboratory study run in 2010 allowed participants to assess the performance of the NAT methods applied in their seasonal routine screening of blood donations.
Authors: Lisa N Pealer; Anthony A Marfin; Lyle R Petersen; Robert S Lanciotti; Peter L Page; Susan L Stramer; Mary Grace Stobierski; Kimberly Signs; Bruce Newman; Hema Kapoor; Jesse L Goodman; Mary E Chamberland Journal: N Engl J Med Date: 2003-09-18 Impact factor: 91.245
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Authors: Giada Rossini; Fabrizio Carletti; Licia Bordi; Francesca Cavrini; Paolo Gaibani; Maria P Landini; Anna Pierro; Maria R Capobianchi; Antonino Di Caro; Vittorio Sambri Journal: Emerg Infect Dis Date: 2011-05 Impact factor: 6.883
Authors: Jens Dreier; Tanja Vollmer; Dennis Hinse; Ernst Joachim Heuser; Giulio Pisani; Cornelius Knabbe Journal: Transfus Med Hemother Date: 2015-09-28 Impact factor: 3.747
Authors: Vittorio Sambri; Maria R Capobianchi; Francesca Cavrini; Rémi Charrel; Olivier Donoso-Mantke; Camille Escadafal; Leticia Franco; Paolo Gaibani; Ernest A Gould; Matthias Niedrig; Anna Papa; Anna Pierro; Giada Rossini; Andrea Sanchini; Antonio Tenorio; Stefania Varani; Ana Vázquez; Caterina Vocale; Herve Zeller Journal: Viruses Date: 2013-09-25 Impact factor: 5.048