Literature DB >> 22030807

Immunohistochemical analysis of collagen content and types in the rectus abdominis muscle of cadavers of different ages.

Eliziane Nitz de Carvalho Calvi1, Fábio Xerfan Nahas, Marcus Vinícius Jardini Barbosa, Silvia Saiuli Miki Ihara, José Augusto Calil, Lydia Masako Ferreira.   

Abstract

PURPOSE: To assess the collagen content and types in the rectus abdominis muscle of cadavers of different ages.
METHODS: Forty fresh adult male cadavers, at room temperature, were obtained from the Institute of Legal Medicine of Franca and dissected within 24 hours of death. The cadavers were divided into two groups: Group A (n=20), 18 to 30 years of age, and Group B (n=20), 31 to 60 years of age. Bilateral incisions were made in the middle portion of anterior rectus sheath 3 cm superiorly and 2 cm inferiorly to the umbilicus and four fragments of the rectus abdominis muscle were dissected. The samples were fixed in 10% buffered formalin and sent for immunohistochemical analysis to determine collagen content and types.
RESULTS: Immunohistochemical results revealed higher amounts of type I and type III collagen in Group A. However, no difference in the amount of type IV collagen was found between the groups.
CONCLUSION: The amount of type I and type III collagen was higher in group A.

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Year:  2011        PMID: 22030807     DOI: 10.1590/s0102-86502011000800002

Source DB:  PubMed          Journal:  Acta Cir Bras        ISSN: 0102-8650            Impact factor:   1.388


  7 in total

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  7 in total

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