BACKGROUND: RECK is a tumor suppressor which inhibits metastasis and angiogenesis. Based on RECK expression in prostate cancer tissue and cell lines, our aim was to investigate functional relevance of RECK for prostate carcinoma. METHODS: RECK protein levels were determined by Western blotting in the human prostate cell lines BPH-1, DU-145, LNCaP, PC-3, and in tissue of 12 normal/tumor matches of patients after radical prostatectomy. Functional characteristics of DU-145 cells with stable RECK overexpression included proliferation, invasion, regulation of matrix metalloproteinases MMP-2, MMP-9, and MMP-14 measured by zymography (MMP-2 and -9) or commercially available assays. RESULTS: RECK was expressed in cell lines and tissue with a significant decrease in malignant tissue (P = 0.002). RECK overexpression caused an up to 80% decrease in invasion for DU-145 cells (P < 0.001) and a decrease of pro-MMP-9 (42%) and of pro-/active MMP-14 (up to 53% of control). Proliferation was not affected by RECK overexpression. CONCLUSIONS: The considerable anti-invasive potential of RECK points to new therapeutic possibilities for prostate cancer.
BACKGROUND:RECK is a tumor suppressor which inhibits metastasis and angiogenesis. Based on RECK expression in prostate cancer tissue and cell lines, our aim was to investigate functional relevance of RECK for prostate carcinoma. METHODS:RECK protein levels were determined by Western blotting in the human prostate cell lines BPH-1, DU-145, LNCaP, PC-3, and in tissue of 12 normal/tumor matches of patients after radical prostatectomy. Functional characteristics of DU-145 cells with stable RECK overexpression included proliferation, invasion, regulation of matrix metalloproteinases MMP-2, MMP-9, and MMP-14 measured by zymography (MMP-2 and -9) or commercially available assays. RESULTS:RECK was expressed in cell lines and tissue with a significant decrease in malignant tissue (P = 0.002). RECK overexpression caused an up to 80% decrease in invasion for DU-145 cells (P < 0.001) and a decrease of pro-MMP-9 (42%) and of pro-/active MMP-14 (up to 53% of control). Proliferation was not affected by RECK overexpression. CONCLUSIONS: The considerable anti-invasive potential of RECK points to new therapeutic possibilities for prostate cancer.
Authors: Andrew Prendergast; Tor H Linbo; Tanya Swarts; Josette M Ungos; Hillary F McGraw; Shlomo Krispin; Brant M Weinstein; David W Raible Journal: Development Date: 2012-02-01 Impact factor: 6.868
Authors: Nijaguna B Prasad; Anne C Fischer; Alice Y Chuang; Jerry M Wright; Ting Yang; Hua-Ling Tsai; William H Westra; Nanette J Liegeois; Allan D Hess; Anthony P Tufaro Journal: Mod Pathol Date: 2013-12-20 Impact factor: 7.842
Authors: L A Walsh; D M Roy; M Reyngold; D Giri; A Snyder; S Turcan; C R Badwe; J Lyman; J Bromberg; T A King; T A Chan Journal: Oncogene Date: 2014-06-16 Impact factor: 9.867