Literature DB >> 22020065

NKX2-5(eGFP/w) hESCs for isolation of human cardiac progenitors and cardiomyocytes.

David A Elliott1, Stefan R Braam, Katerina Koutsis, Elizabeth S Ng, Robert Jenny, Ebba L Lagerqvist, Christine Biben, Tanya Hatzistavrou, Claire E Hirst, Qing C Yu, Rhys J P Skelton, Dorien Ward-van Oostwaard, Sue Mei Lim, Ouda Khammy, Xueling Li, Susan M Hawes, Richard P Davis, Adam L Goulburn, Robert Passier, Owen W J Prall, John M Haynes, Colin W Pouton, David M Kaye, Christine L Mummery, Andrew G Elefanty, Edouard G Stanley.   

Abstract

NKX2-5 is expressed in the heart throughout life. We targeted eGFP sequences to the NKX2-5 locus of human embryonic stem cells (hESCs); NKX2-5(eGFP/w) hESCs facilitate quantification of cardiac differentiation, purification of hESC-derived committed cardiac progenitor cells (hESC-CPCs) and cardiomyocytes (hESC-CMs) and the standardization of differentiation protocols. We used NKX2-5 eGFP(+) cells to identify VCAM1 and SIRPA as cell-surface markers expressed in cardiac lineages.

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Year:  2011        PMID： 22020065     DOI： 10.1038/nmeth.1740

Source DB:  PubMed          Journal:  Nat Methods        ISSN： 1548-7091            Impact factor:   28.547


  29 in total

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Journal:  Nat Protoc       Date:  2007       Impact factor: 13.491

2.  A protocol for removal of antibiotic resistance cassettes from human embryonic stem cells genetically modified by homologous recombination or transgenesis.

Authors:  Richard P Davis; Magdaline Costa; Catarina Grandela; Andrew M Holland; Tanya Hatzistavrou; Suzanne J Micallef; Xueling Li; Adam L Goulburn; Lisa Azzola; Andrew G Elefanty; Edouard G Stanley
Journal:  Nat Protoc       Date:  2008       Impact factor: 13.491

3.  A bacterial artificial chromosome library for sequencing the complete human genome.

Authors:  K Osoegawa; A G Mammoser; C Wu; E Frengen; C Zeng; J J Catanese; P J de Jong
Journal:  Genome Res       Date:  2001-03       Impact factor: 9.043

4.  Embryonic stem cell lines from human blastocysts: somatic differentiation in vitro.

Authors:  B E Reubinoff; M F Pera; C Y Fong; A Trounson; A Bongso
Journal:  Nat Biotechnol       Date:  2000-04       Impact factor: 54.908

5.  A protocol describing the use of a recombinant protein-based, animal product-free medium (APEL) for human embryonic stem cell differentiation as spin embryoid bodies.

Authors:  Elizabeth S Ng; Richard Davis; Edouard G Stanley; Andrew G Elefanty
Journal:  Nat Protoc       Date:  2008       Impact factor: 13.491

6.  Cardiomyocytes derived from human embryonic stem cells in pro-survival factors enhance function of infarcted rat hearts.

Authors:  Michael A Laflamme; Kent Y Chen; Anna V Naumova; Veronica Muskheli; James A Fugate; Sarah K Dupras; Hans Reinecke; Chunhui Xu; Mohammad Hassanipour; Shailaja Police; Chris O'Sullivan; Lila Collins; Yinhong Chen; Elina Minami; Edward A Gill; Shuichi Ueno; Chun Yuan; Joseph Gold; Charles E Murry
Journal:  Nat Biotechnol       Date:  2007-08-26       Impact factor: 54.908

7.  Human cardiovascular progenitor cells develop from a KDR+ embryonic-stem-cell-derived population.

Authors:  Lei Yang; Mark H Soonpaa; Eric D Adler; Torsten K Roepke; Steven J Kattman; Marion Kennedy; Els Henckaerts; Kristina Bonham; Geoffrey W Abbott; R Michael Linden; Loren J Field; Gordon M Keller
Journal:  Nature       Date:  2008-04-23       Impact factor: 49.962

8.  Mouse ES cell-derived cardiac precursor cells are multipotent and facilitate identification of novel cardiac genes.

Authors:  Nicolas Christoforou; Ronald A Miller; Christine M Hill; Chunfa C Jie; Andrew S McCallion; John D Gearhart
Journal:  J Clin Invest       Date:  2008-03       Impact factor: 14.808

9.  Differentiation in vivo of cardiac committed human embryonic stem cells in postmyocardial infarcted rats.

Authors:  André Tomescot; Julia Leschik; Valérie Bellamy; Gilbert Dubois; Emmanuel Messas; Patrick Bruneval; Michel Desnos; Albert A Hagège; Michal Amit; Joseph Itskovitz; Philippe Menasché; Michel Pucéat
Journal:  Stem Cells       Date:  2007-05-31       Impact factor: 6.277

10.  Nkx-2.5: a novel murine homeobox gene expressed in early heart progenitor cells and their myogenic descendants.

Authors:  T J Lints; L M Parsons; L Hartley; I Lyons; R P Harvey
Journal:  Development       Date:  1993-10       Impact factor: 6.868
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  183 in total

Review 1.  Find and replace: editing human genome in pluripotent stem cells.

Authors:  Huize Pan; Weiqi Zhang; Weizhou Zhang; Guang-Hui Liu
Journal:  Protein Cell       Date:  2011-12-15       Impact factor: 14.870

2.  Recognizing heart cells in a crowd.

Authors:  Timothy J Kamp
Journal:  Nat Methods       Date:  2011-11-29       Impact factor: 28.547

3.  Robust cardiomyocyte differentiation from human pluripotent stem cells via temporal modulation of canonical Wnt signaling.

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Journal:  Proc Natl Acad Sci U S A       Date:  2012-05-29       Impact factor: 11.205

4.  Cardiac differentiation of human pluripotent stem cells in scalable suspension culture.

Authors:  Henning Kempf; Christina Kropp; Ruth Olmer; Ulrich Martin; Robert Zweigerdt
Journal:  Nat Protoc       Date:  2015-08-13       Impact factor: 13.491

5.  Purification of cardiomyocytes from differentiating pluripotent stem cells using molecular beacons that target cardiomyocyte-specific mRNA.

Authors:  Kiwon Ban; Brian Wile; Sangsung Kim; Hun-Jun Park; Jaemin Byun; Kyu-Won Cho; Talib Saafir; Ming-Ke Song; Shan Ping Yu; Mary Wagner; Gang Bao; Young-Sup Yoon
Journal:  Circulation       Date:  2013-08-30       Impact factor: 29.690

6.  Productive Infection of Human Embryonic Stem Cell-Derived NKX2.1+ Respiratory Progenitors with Human Rhinovirus.

Authors:  Robert A Jenny; Claire Hirst; Sue Mei Lim; Adam L Goulburn; Suzanne J Micallef; Tanya Labonne; Anthony Kicic; Kak-Ming Ling; Stephen M Stick; Elizabeth S Ng; Alan Trounson; Antonietta Giudice; Andrew G Elefanty; Edouard G Stanley
Journal:  Stem Cells Transl Med       Date:  2015-04-14       Impact factor: 6.940

Review 7.  The non-coding road towards cardiac regeneration.

Authors:  James E Hudson; Enzo R Porrello
Journal:  J Cardiovasc Transl Res       Date:  2013-12       Impact factor: 4.132

8.  Design and formulation of functional pluripotent stem cell-derived cardiac microtissues.

Authors:  Nimalan Thavandiran; Nicole Dubois; Alexander Mikryukov; Stéphane Massé; Bogdan Beca; Craig A Simmons; Vikram S Deshpande; J Patrick McGarry; Christopher S Chen; Kumaraswamy Nanthakumar; Gordon M Keller; Milica Radisic; Peter W Zandstra
Journal:  Proc Natl Acad Sci U S A       Date:  2013-11-19       Impact factor: 11.205

9.  A human embryonic stem cell reporter line for monitoring chemical-induced cardiotoxicity.

Authors:  Su-Yi Tsai; Zaniar Ghazizadeh; Hou-Jun Wang; Sadaf Amin; Francis A Ortega; Zohreh Sadat Badieyan; Zi-Ting Hsu; Miriam Gordillo; Ritu Kumar; David J Christini; Todd Evans; Shuibing Chen
Journal:  Cardiovasc Res       Date:  2020-03-01       Impact factor: 10.787

Review 10.  Induced pluripotent stem cells: the new patient?

Authors:  Milena Bellin; Maria C Marchetto; Fred H Gage; Christine L Mummery
Journal:  Nat Rev Mol Cell Biol       Date:  2012-10-04       Impact factor: 94.444
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