M Rowshahul Habib1, M Rezaul Karim. 1. Department of Biochemistry and Molecular Biology, Rajshahi University, Rajshahi-6205, Bangladesh.
Abstract
OBJECTIVE: To investigate experimentally the possible antitumor effect of methanol extract (ME) of Calotropis gigantea L. (C. gigantean) root bark and its petroleum ether (PEF) and chloroform (CF) soluble fractions against Ehrlich ascites carcinoma (EAC) in Swiss albino mice. METHODS: The effects of ME (10 and 20 mg/kg), PEF (40 and 80 mg/kg) and CF (20 and 40 mg/kg) on the growth of EAC and life span of EAC bearing mice were studied. Hematological profile and biochemical parameters (SALP, SGPT and SGOT) were also estimated. RESULTS: Results of in vivo study showed a significant decrease in viable tumor cell count and a significant increase of life span in the ME and CF treated group compared to untreated one. The life span of ME and CF treated animals was significantly (P<0.05) increased by 43.90% (20 mg ME/kg) and 57.07% (40 mg CF/kg). ME and CF brought back the hematological parameter more or less normal level. ME and CF also restored the altered levels of serum alkaline phosphatase (SALP) and serum glutamate oxaloacetate transaminase (SGOT). CONCLUSIONS: Methanol extract (ME) of C. gigantea root bark and its chloroform soluble fraction (CF) possesses significant antitumor activity.
OBJECTIVE: To investigate experimentally the possible antitumor effect of methanol extract (ME) of Calotropis gigantea L. (C. gigantean) root bark and its petroleum ether (PEF) and chloroform (CF) soluble fractions against Ehrlich ascites carcinoma (EAC) in Swiss albino mice. METHODS: The effects of ME (10 and 20 mg/kg), PEF (40 and 80 mg/kg) and CF (20 and 40 mg/kg) on the growth of EAC and life span of EAC bearing mice were studied. Hematological profile and biochemical parameters (SALP, SGPT and SGOT) were also estimated. RESULTS: Results of in vivo study showed a significant decrease in viable tumor cell count and a significant increase of life span in the ME and CF treated group compared to untreated one. The life span of ME and CF treated animals was significantly (P<0.05) increased by 43.90% (20 mg ME/kg) and 57.07% (40 mg CF/kg). ME and CF brought back the hematological parameter more or less normal level. ME and CF also restored the altered levels of serum alkaline phosphatase (SALP) and serum glutamate oxaloacetate transaminase (SGOT). CONCLUSIONS:Methanol extract (ME) of C. gigantea root bark and its chloroform soluble fraction (CF) possesses significant antitumor activity.
Authors: Shaikh M Mohsin Ali; M Abul Kalam Azad; Mele Jesmin; Shamim Ahsan; M Mijanur Rahman; Jahan Ara Khanam; M Nazrul Islam; Sha M Shahan Shahriar Journal: Asian Pac J Trop Biomed Date: 2012-06
Authors: Cristina M Villanueva; Esther Gracia-Lavedan; Cristina Bosetti; Elena Righi; Antonio José Molina; Vicente Martín; Elena Boldo; Nuria Aragonés; Beatriz Perez-Gomez; Marina Pollan; Ines Gomez Acebo; Jone M Altzibar; Ana Jiménez Zabala; Eva Ardanaz; Rosana Peiró; Adonina Tardón; Maria Dolores Chirlaque; Alessandra Tavani; Jerry Polesel; Diego Serraino; Federica Pisa; Gemma Castaño-Vinyals; Ana Espinosa; Nadia Espejo-Herrera; Margarita Palau; Victor Moreno; Carlo La Vecchia; Gabriella Aggazzotti; Mark J Nieuwenhuijsen; Manolis Kogevinas Journal: Environ Health Perspect Date: 2016-07-06 Impact factor: 9.031