OBJECTIVE: To identify inhibitors of nitric oxide production and NF-κB activity from Chromolaena odorata (C. odorata). METHODS: The compounds isolated from the aerial parts of C. odorata by bioassay-guided fractionation were investigated for their inhibitory effects on the NO production and NF-κB activity in LPS-stimulated RAW264.7 cells. RESULTS: Six fatty acids (S)-coriolic acid (1), (S)-coriolic acid methyl ester (2), (S)-15,16-didehydrocoriolic acid (3), (S)-15,16-didehydrocoriolic acid methyl ester (4), linoleamide (5) and linolenamide (6) were isolated. All compounds inhibited the NO production at concentrations consistent with those required for NF-κB inhibition. Compound 2 was the most active with the IC(50) values of 5.22 and 5.73 μM. The addition of a double bond in the fatty chain decreased the inhibitory effects while the methyl esterification increased the activities. CONCLUSIONS: The fatty acid components in C. odorata with NF-κB inhibitory activity could explain the anti-inflammation property of this plant in traditional medicine. This study could also contribute to the better use of C. odorata for human health care.
OBJECTIVE: To identify inhibitors of nitric oxide production and NF-κB activity from Chromolaena odorata (C. odorata). METHODS: The compounds isolated from the aerial parts of C. odorata by bioassay-guided fractionation were investigated for their inhibitory effects on the NO production and NF-κB activity in LPS-stimulated RAW264.7 cells. RESULTS: Six fatty acids(S)-coriolic acid (1), (S)-coriolic acid methyl ester (2), (S)-15,16-didehydrocoriolic acid (3), (S)-15,16-didehydrocoriolic acid methyl ester (4), linoleamide (5) and linolenamide (6) were isolated. All compounds inhibited the NO production at concentrations consistent with those required for NF-κB inhibition. Compound 2 was the most active with the IC(50) values of 5.22 and 5.73 μM. The addition of a double bond in the fatty chain decreased the inhibitory effects while the methyl esterification increased the activities. CONCLUSIONS: The fatty acid components in C. odorata with NF-κB inhibitory activity could explain the anti-inflammation property of this plant in traditional medicine. This study could also contribute to the better use of C. odorata for human health care.
Authors: Thi Van Anh Tran; Clemens Malainer; Stefan Schwaiger; Tran Hung; Atanas G Atanasov; Elke H Heiss; Verena M Dirsch; Hermann Stuppner Journal: J Ethnopharmacol Date: 2014-10-31 Impact factor: 4.360
Authors: Hajo Idriss; Babeker Siddig; Pamela González Maldonado; H M Elkhair; A I Alakhras; Emad M Abdallah; Pablo Hernán Sotelo Torres; Amin O Elzupir Journal: Molecules Date: 2022-08-01 Impact factor: 4.927
Authors: Elke H Heiss; Thi Van Anh Tran; Kristin Zimmermann; Stefan Schwaiger; Corina Vouk; Barbara Mayerhofer; Clemens Malainer; Atanas G Atanasov; Hermann Stuppner; Verena M Dirsch Journal: J Nat Prod Date: 2014-01-29 Impact factor: 4.050