PURPOSE: To evaluate the effect of a biodegradable microfilm with sustained release of prednisolone acetate (PA) on postoperative wound healing after experimental glaucoma filtration surgery (GFS). METHODS: Biodegradable microfilms composed of poly (D-, L-lactide-co-caprolactone) (PLC) were fabricated and then pre-loaded PA-20% total weight. Fourteen New Zealand White rabbits were randomly divided into 3 treatment groups: GFS alone (n=4), GFS with PLC microfilms (n=4) and GFS with PA-loaded microfilm (n=6). Microfilms were inserted subconjunctivally, adjacent to the filtering surgical site. We monitored all eyes with slit-lamp examination, bleb photography and anterior segment optical coherence tomography (AS-OCT). Histology with immunohistochemistry was performed to determine the presence of any inflammation. RESULTS: Prednisolone acetate 20%-loaded microfilms exhibited steady, sustained release in vitro. Eyes implanted with PA-loaded microfilms showed a significantly better bleb survival (100% vs. 37.5%, p<0.001) and reduced bleb vascularity (58%; 95% CI 54-62% vs. 30%; 95% CI 23-37%, p=0.001) compared to the control at 30 days postoperatively. Histology and immunohistochemistry demonstrated less T-cell infiltration in the eye implanted with PA-loaded microfilms. CONCLUSION: Subconjunctival insertion of a PA-loaded biodegradeable microfilm exhibit sustained release of PA to reduce postoperative inflammation and prolong bleb survival in rabbit GFS.
PURPOSE: To evaluate the effect of a biodegradable microfilm with sustained release of prednisolone acetate (PA) on postoperative wound healing after experimental glaucoma filtration surgery (GFS). METHODS: Biodegradable microfilms composed of poly (D-, L-lactide-co-caprolactone) (PLC) were fabricated and then pre-loaded PA-20% total weight. Fourteen New Zealand White rabbits were randomly divided into 3 treatment groups: GFS alone (n=4), GFS with PLC microfilms (n=4) and GFS with PA-loaded microfilm (n=6). Microfilms were inserted subconjunctivally, adjacent to the filtering surgical site. We monitored all eyes with slit-lamp examination, bleb photography and anterior segment optical coherence tomography (AS-OCT). Histology with immunohistochemistry was performed to determine the presence of any inflammation. RESULTS:Prednisolone acetate 20%-loaded microfilms exhibited steady, sustained release in vitro. Eyes implanted with PA-loaded microfilms showed a significantly better bleb survival (100% vs. 37.5%, p<0.001) and reduced bleb vascularity (58%; 95% CI 54-62% vs. 30%; 95% CI 23-37%, p=0.001) compared to the control at 30 days postoperatively. Histology and immunohistochemistry demonstrated less T-cell infiltration in the eye implanted with PA-loaded microfilms. CONCLUSION: Subconjunctival insertion of a PA-loaded biodegradeable microfilm exhibit sustained release of PA to reduce postoperative inflammation and prolong bleb survival in rabbit GFS.
Authors: Marcus Ang; Xuwen Ng; Cheewai Wong; Peng Yan; Soon-Phaik Chee; Subbu S Venkatraman; Tina T Wong Journal: PLoS One Date: 2014-05-19 Impact factor: 3.240
Authors: Yu-Chi Liu; Anthony Herr Cheun Ng; Xu Wen Ng; Peng Yan; Subbu S Venkatraman; Jodhbir S Mehta; Tina T Wong Journal: Transl Vis Sci Technol Date: 2017-10-05 Impact factor: 3.283
Authors: Yu-Chi Liu; Yan Peng; Nyein Chan Lwin; Subbu S Venkatraman; Tina T Wong; Jodhbir S Mehta Journal: PLoS One Date: 2013-08-05 Impact factor: 3.240