Tsui Hsien Huang1, Yu Chuan Lu, Chia Tze Kao. 1. Graduate School of Dentistry, Chung Shan Medical University Hospital, Chung Shan Medical University, 110, section 1, Chien Kuo N Road, Taichung City, 40201, Taiwan.
Abstract
UNLABELLED: In this study, the aim is to investigate the cytologic effects of inflammatory bone cells after in vitro low-level laser therapy (LLLT). A human osteosarcoma cell line (MG63) was cultured, infected with lipopolysaccharide (LPS) and exposed to low-level laser treatment at 5 or 10 J/cm(2) using a 920 nm diode laser. MG63 cell attachment was observed under a microscope, and cell viability was quantified by mitochondrial colorimetric assay (MTT). LPS-treated MG63 cells were irradiated with LLLT, and the inflammatory markers iNOS, TNF-α and IL-1, were analyzed by reverse transcription polymerase chain reaction (RT-PCR) and Western blot. The data were collected and analyzed by one-way analysis of variance (ANOVA); p < 0.05 indicated a statistically significant difference. Low-level laser treatment on MG63 cells increased their ability to attach and survive. After irradiation, the expression levels of iNOS, TNF-α and IL-1 in LPS-infected MG63 cells decreased over time (p < 0.05). CONCLUSIONS: low-level diode laser treatment increased the MG63 cell proliferative ability and decreased the expression of inflammatory mediators in MG63 cells.
UNLABELLED: In this study, the aim is to investigate the cytologic effects of inflammatory bone cells after in vitro low-level laser therapy (LLLT). A humanosteosarcoma cell line (MG63) was cultured, infected with lipopolysaccharide (LPS) and exposed to low-level laser treatment at 5 or 10 J/cm(2) using a 920 nm diode laser. MG63 cell attachment was observed under a microscope, and cell viability was quantified by mitochondrial colorimetric assay (MTT). LPS-treated MG63 cells were irradiated with LLLT, and the inflammatory markers iNOS, TNF-α and IL-1, were analyzed by reverse transcription polymerase chain reaction (RT-PCR) and Western blot. The data were collected and analyzed by one-way analysis of variance (ANOVA); p < 0.05 indicated a statistically significant difference. Low-level laser treatment on MG63 cells increased their ability to attach and survive. After irradiation, the expression levels of iNOS, TNF-α and IL-1 in LPS-infected MG63 cells decreased over time (p < 0.05). CONCLUSIONS: low-level diode laser treatment increased the MG63 cell proliferative ability and decreased the expression of inflammatory mediators in MG63 cells.
Authors: Dritan Turhani; Martina Scheriau; David Kapral; Thomas Benesch; Erwin Jonke; Hans Peter Bantleon Journal: Am J Orthod Dentofacial Orthop Date: 2006-09 Impact factor: 2.650
Authors: Martin Parizek; Timothy E L Douglas; Katarina Novotna; Alexander Kromka; Mariea A Brady; Andrea Renzing; Eske Voss; Marketa Jarosova; Lukas Palatinus; Pavel Tesarek; Pavla Ryparova; Věra Lisa; Ana M dos Santos; Patrick H Warnke; Lucie Bacakova Journal: Int J Nanomedicine Date: 2012-04-17