Literature DB >> 21997084

Three-dimensional Fluorescence Lifetime Imaging with a Single Plane Illumination Microscope provides an improved signal to noise ratio.

Klaus Greger1, Manuel J Neetz, Emmanuel G Reynaud, Ernst H K Stelzer.   

Abstract

We designed a widefield frequency domain Fluorescence Lifetime Imaging Microscopy (FLIM)setup, which is based on a Single Plane Illumination Microscope (SPIM). A SPIM provides an inherent optical sectioning capability and reduces photobleaching compared to conventional widefield and confocal fluorescence microscopes. The lifetime precision of the FLIM was characterized with Rhodamine 6G solutions of different quencher concentrations [KI]. We demonstrate the high spatial resolution of the SPIM-FLIM combination in the intensity domain as well as in the lifetime domain with latex bead samples and multiple recordings of three-dimensional live Madine-Darby Canine Kidney (MDCK) cysts. We estimate that the bleaching rate after 600 images have been recorded is below 5%.

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Year:  2011        PMID： 21997084     DOI： 10.1364/OE.19.020743

Source DB:  PubMed          Journal:  Opt Express        ISSN： 1094-4087            Impact factor:   3.894


  10 in total

1.  Imaging fluorescence (cross-) correlation spectroscopy in live cells and organisms.

Authors:  Jan W Krieger; Anand P Singh; Nirmalya Bag; Christoph S Garbe; Timothy E Saunders; Jörg Langowski; Thorsten Wohland
Journal:  Nat Protoc       Date:  2015-11-05       Impact factor: 13.491

2.  Light-sheet fluorescence microscopy for quantitative biology.

Authors:  Ernst H K Stelzer
Journal:  Nat Methods       Date:  2015-01       Impact factor: 28.547

3.  csiLSFM combines light-sheet fluorescence microscopy and coherent structured illumination for a lateral resolution below 100 nm.

Authors:  Bo-Jui Chang; Victor Didier Perez Meza; Ernst H K Stelzer
Journal:  Proc Natl Acad Sci U S A       Date:  2017-04-24       Impact factor: 11.205

4.  Parallel excitation-emission multiplexed fluorescence lifetime confocal microscopy for live cell imaging.

Authors:  Ming Zhao; Yu Li; Leilei Peng
Journal:  Opt Express       Date:  2014-05-05       Impact factor: 3.894

Review 5.  Optical probes and techniques for O2 measurement in live cells and tissue.

Authors:  Ruslan I Dmitriev; Dmitri B Papkovsky
Journal:  Cell Mol Life Sci       Date:  2012-01-17       Impact factor: 9.261

6.  Selective plane illumination microscopy (SPIM) with time-domain fluorescence lifetime imaging microscopy (FLIM) for volumetric measurement of cleared mouse brain samples.

Authors:  Tsukasa Funane; Steven S Hou; Katarzyna Marta Zoltowska; Susanne J van Veluw; Oksana Berezovska; Anand T N Kumar; Brian J Bacskai
Journal:  Rev Sci Instrum       Date:  2018-05       Impact factor: 1.523

7.  Luminescence lifetime imaging of three-dimensional biological objects.

Authors:  Ruslan I Dmitriev; Xavier Intes; Margarida M Barroso
Journal:  J Cell Sci       Date:  2021-05-07       Impact factor: 5.285

8.  Fluorescence lifetime imaging microscopy: fundamentals and advances in instrumentation, analysis, and applications.

Authors:  Rupsa Datta; Tiffany M Heaster; Joe T Sharick; Amani A Gillette; Melissa C Skala
Journal:  J Biomed Opt       Date:  2020-05       Impact factor: 3.170

9.  Lightsheet fluorescence lifetime imaging microscopy with wide-field time-correlated single photon counting.

Authors:  Liisa M Hirvonen; Jakub Nedbal; Norah Almutairi; Thomas A Phillips; Wolfgang Becker; Thomas Conneely; James Milnes; Susan Cox; Stephen Stürzenbaum; Klaus Suhling
Journal:  J Biophotonics       Date:  2019-11-25       Impact factor: 3.207

Review 10.  High-throughput, multi-parametric, and correlative fluorescence lifetime imaging.

Authors:  Chetan Poudel; Ioanna Mela; Clemens F Kaminski
Journal:  Methods Appl Fluoresc       Date:  2020-02-20       Impact factor: 3.009
  10 in total

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