Cerebellar and visual gray matter brain volume increases in congenital nystagmus.

Structural brain abnormalities associated with congenital nystagmus (CN) are still unknown. In some patients with CN additional sensory, metabolic, or gross structural alterations can be detected. In the present study voxel-based morphometry was used to compare the gray matter (GM) brain volumes of 14 individuals with CN without associated sensory, metabolic, or obvious structural alterations (i.e., idiopathic CN) to those of a group of controls. Further, GM brain volumes were correlated with nystagmus severity as measured by sway path. Intergroup comparison exhibited significant volume increases in the human motion sensitive complex V5/MT+, the fusiform gyrus, and the middle occipital gyrus bilaterally in CN. These volume increases may be associated with excess visual motion stimulation due to involuntary retinal slip of the visual scene. A positive correlation (linear model) of nystagmus sway path with cerebellar GM volume was seen in the following areas: vermal parts VIII-X as well as hemisphere lobule II, hemisphere VI, crus I, crus II, and lobule VII-IX bilaterally. There is evidence that the reported GM volume changes in the vestibulo-cerebellum, which correlated with nystagmus sway path, might be related to the subjects' attempt to maintain fixation, rather than be due to the generation of nystagmus.

Introduction

Congenital nystagmus (CN; classified as infant nystagmus syndrome in 2001) has its onset within the first few months of life. In some individuals it may be associated with metabolic (e.g., hypothyroidism), sensory (e.g., achromatopsia), or structural abnormalities of the brain (excessive or absent crossing of nasal fibers at the optic chiasm; Leigh and Zee, 1999). In over 60% of afflicted subjects no additional abnormalities can be found (Leigh and Zee, 1999), this entity can thus be termed “idiopathic CN.” The clinical picture of CN can be highly variable, but its typical appearance is that of a conjugate, predominantly horizontally beating jerk nystagmus with waveforms that show a slow phase with an exponential increase in velocity or that have a pendular shape; periods of foveation are frequently superimposed on both waveforms (Jacobs and Dell’Osso, 2004). The nystagmus interferes with fixation, is often exacerbated in stressful situations, and is sometimes suppressed by convergence. Intensity varies depending on gaze direction (null zone). Individuals with CN can have normal or near normal visual function (depending on foveation times) unless additional afferent deficits are present (for an overview of clinical characteristics see Khanna and Dell’Osso, 2006). Unlike patients with acquired nystagmus, most individuals with CN do not experience illusory motion of the visual surroundings (oscillopsia; Leigh et al., 1988). Such spatial inconstancy can, however, be provoked in many CN individuals under laboratory conditions, depending on the contrast and luminance of the stimulus as well as the viewing angle (Tkalcevic and Abel, 2003).

Several compelling theories have been put forward to elucidate the pathophysiology of abnormal eye movements in CN. All these models and hypotheses have been generated on the basis of eye movement analysis. Voxel-based morphometry (VBM) has not yet been used (to the best of our knowledge) to approach the question of whether CN might be associated with distinct brain volume changes. Our aim in this study was thus to identify such brain areas using VBM. On the basis of previous literature, the region of interest (ROI) selected included the cerebellum and the visually driven cortex areas (Schmitz et al., 2004; Schlindwein et al., 2009).

Materials and Methods

Subjects

Fourteen individuals (seven males, mean age: 28.3 years, SD: 9.38 years) diagnosed to have CN at the Department of Ophthalmology, Ludwig-Maximilians University, Munich, Germany were included in the study (four additional individuals with CN had to be excluded from the study after on-site questioning revealed incompatibilities for elective MRI scanning). All individuals had undergone surgery for correction of CN 1–29 years prior to the study at the Department of Ophthalmology, Ludwig-Maximilians University, Munich, Germany. Subject characteristics are given in Table 1; all subjects had conjugate nystagmus and eight subjects had amblyopia. No further ophthalmological disorders were noted. Subjects were compared to a group of 18 individuals without any ocular motor, neurological, or visual abnormalities (11 males, mean age: 28.56 year, SD: 8.58 years). In accordance with the Declaration of Helsinki (last adopted at the 52nd World Medical Association General Assembly 2000 in Edinburgh), all subjects gave their informed, written consent before the experiment. The study was approved by the Ethics Committee of the Ludwig-Maximilians University, Munich, Germany.

Table 1

Subject characteristics.

Subject ID	Latent component	VA logMAR	Sway path max nystagmus (°/s)	Angle (°)	Surgery	Ocular alignment	Amblyopia
CN01	Minimal	R: 0.12/L: 0.18	124	−22	2009	Orthophoria	Minimal, both eyes
CN02	No	R: 0.23/L: 0	32	−20	1992	Exotropia, −VD	Minimal, R eye
CN03	n.e.	n.e.	19	−4	n.e.	n.e.	n.e.
CN04	No	R: 0.08/L: 0.12	62	4	1995/2005	−VD	No
CN05	No	R: 0.23/L: 0.23	51	−7	1999	Orthophoria	Minimal, both eyes
CN06	No	R: 0/L: 0	58	−18	1996	Exophoria	No
CN07	No	R: 0.48/L: 0.38	17	7	1984/1994	Exophoria, +VD	Minimal, both eyes
CN08	No	R: 0.6/L: 0.12	14	−17	1996	Microstrabismus right eye	R eye
CN09	No	R: 0.08/L: 0.48	51	8	2001	Orthophoria	No
CN10	No	R: 0/L: 1.5	6	0	2004/2005	Orthophoria	L eye
CN11	No	R: 0/L: 0	74	−8	1999/2000	Orthophoria	No
CN12	No	R: 0.23/L: 0.18	83	12	1993/2004	Exophoria	No
CN13	Left eye	R: 0.12/L: 0.3	17	20	1998	Esotropia	Minimal, L eye
CN14	Both eyes	R: 0.78/L: 0	26	−7	1980	−VD	R eye

All visual acuities were measured in the preferred head position of the subject with correction where necessary at a distance of 5 m. “Sway path” was used to express the absolute value of eye velocity, i.e., the length of the path of gaze in vertical and horizontal directions for a period of time. Sway path was used as a measure of total retinal slip. Sway path is irrespective of the direction of beat. “Angle” denotes the tilt of the principal component of eye movements with respect to the horizontal direction for the maximal nystagmus condition and is expressed as a deviation from the horizontal direction which is set to 0. For positive values the tilt is counterclockwise (from the subjects point of view), and clockwise for negative values. Only surgeries related to CN and the shift of the null zone are listed. N.e., not evaluated; VD, vertical divergence.

MRI measurements

Acquisition protocol

All MRI scans were obtained using a clinical standard 3.0 T General Electric (GE) Signa system (HDx platform; GE Medical Systems, Milwaukee, WI, USA) and an eight-channel standard head coil. A 3D gradient-echo sequence (FSPGR fast-spoiled gradient recalled), with a voxel size of 0.86 mm × 0.86 mm × 1.4 mm, and 0.7 mm oversampling in the z direction was used to acquire T1-weighted brain images from all subjects.

Voxel-based morphometry

Voxel-based morphometry was used to analyze the 3D FSPGR data sets. Data were processed using SPM5 (Wellcome Department of Cognitive Neurology, London, UK) and the VBM 5.1 toolbox, both implemented in Matlab (The MathWorks, Natick, MA, USA). The following preprocessing steps of the VBM toolbox were applied: spatial normalization to the space defined by the MNI template, tissue classification, and registration using linear and non-linear transformations (warping) within the same generative model (Ashburner and Friston, 2005). Analysis was performed on modulated gray matter (GM) segments, which were multiplied by the non-linear components derived from the normalization matrix to preserve actual GM volumes locally, and then smoothed with an 8-mm isotropic Gaussian kernel. The confounding effects of individual differences in brain orientation, alignment, and size were accounted for by applying modulation for non-linear warping only.

Voxel-wise GM differences between CN subjects and controls were analyzed using statistical parametric mapping software (SPM5). Voxel-wise GM intensity values for each subject were entered according to their group into a general linear model. Age was included as a covariate in the model. Voxel-wise statistical parametric maps were created using linear contrasts, which identified brain regions containing significant local GM volume differences between CN subjects and controls (Friston et al., 1995). A functional ROI for visual motion processing was created in this specific study population by scanning the same patients and controls that participated in the VBM experiment and by mapping the area V5/MT+ as well as other visually driven cortex areas using a localization fMRI experiment. fMRI stimuli were presented on a back projection screen (visual field of view 25° horizontal, 19° vertical, viewing distance: 60 cm) located inside the bore of the MRI scanner, behind the subject’s head. Subjects were able to view this screen using a mirror. A random dot movement based on experiments by Morrone et al. (2000) was used for visual motion stimulation during fixation of a central target. The motion stimuli consisted of 100 white and 100 black dots moving in random directions (dot diameter 0.3°, speed 7°/s, lifetime 0.3 s, reversal time 2 s) on a gray background. They were presented within a rectangular area that reached from +7° off center to the right border of the screen and from −7° off center to the left border of the screen. The control condition consisted of fixation of a stationary target on an isoluminant gray background. The motion stimuli were alternated with the control condition in a block design (block length 8 MRI scans, TR 2500 ms, six repetitions of each motion stimulus). The data were analyzed for CN individuals and controls together to obtain the mask used as a ROI (including: lingual gyrus, fusiform gyrus, superior, middle and inferior occipital gyrus, calcarine area, superior and inferior temporal gyrus). All VBM results of CN > control and control > CN are reported within this functional ROI.

In a second, separate general linear model analysis, GM volume changes in the cerebellum were correlated with nystagmus sway path and angle using images from only the CN subject group. A ROI covering the entire cerebellum was created using the WFU-Pickatlas toolbox (ANSIR, Wake Forest University; Maldjian et al., 2003). All results of the analysis are reported within this cerebellar ROI. Anatomical structures were named according to the Automated Anatomical Labeling Atlas (Tzourio-Mazoyer et al., 2002) for supratentorial structures and using Schmahmann et al. (1999) and Larsell and Jansen (1972) for the cerebellum. In all analyses, results at p < 0.05 corrected for multiple comparisons, FDR (false discovery rate), were considered significant. Clusters exceeding 1 voxel were reported.

Video-oculography

Eye movements were recorded in all CN subjects using infrared video-oculography (VOG) with an in-house custom-built system (software “EyeSeeCam”; Schneider et al., 2009) that stored horizontal and vertical eye positions, eye velocities, and video recordings. An analog video camera monitored the right eye of the subjects at 60 frames per second while the left eye was covered with a patch. Resolution of the VOG was <0.1°. A five point calibration procedure was performed in all subjects. Eye movements were then recorded during fixation of a target with minimal and maximal nystagmus positions. Analysis of the recorded VOG data was performed off-line using Matlab (The MathWorks). Mean horizontal and vertical eye positions and mean eye velocities were calculated for minimal and maximal nystagmus conditions. The absolute value of eye velocity was expressed as sway path (°/s), i.e., the length of the path of gaze in vertical and horizontal directions for a period of time. Sway path was used as a measure of total retinal slip. Sway path is irrespective of the direction of beat. The predominant beating direction (nystagmus angle) of the nystagmus for the maximal nystagmus condition (degree) was also computed and expressed as a deviation from the horizontal direction.

Results

Intergroup comparison of gray matter volume in CN individuals vs. controls

Larger GM volumes were found in CN individuals in the middle occipital gyrus, the fusiform gyrus, and in the middle/inferior temporal gyrus bilaterally (Table 2; Figure 1A). The volume changes in the middle/inferior temporal gyrus were located within the caudal part of the mapped area for V5/MT+ (Figure 1B). When visual acuity was included as a covariate in the analysis, no additional GM changes were detected (not shown). No GM volume decreases were found in CN individuals compared to controls.

Table 2

Gray matter volume increases in CN subjects vs. controls.

Anatomical location	MNI coordinates			Cluster size in voxels	T-value
	x	y	z
CN SUBJECTS VS. CONTROLS
Mid occipital gyrus L	−41	−72	11	140	4.72
Fusiform gyrus L	−28	−61	−15	712	4.61
Fusiform gyrus L	−26	−52	−14		4.00
Fusiform gyrus L	−34	−52	−10		3.93
Fusiform gyrus R	31	−60	−15	289	4.31
Fusiform gyrus R	28	−49	−10		4.22
Inf temporal gyrus V5/MT+ R	56	−63	−5	224	4.27
Mid occipital gyrus R	31	−85	17	171	4.15
Inf temporal gyrus V5/MT+ L	−53	−67	−7	22	4.07
Mid occipital gyrus L	−32	−87	18	2	3.41

Region of interest analysis, two sample .

Figure 1

Results of the contrast CN subjects vs. controls. (A) The areas of volume increase in CN subjects were projected onto an individual anatomical brain chosen at random. The slices were chosen from z = −12 to z = 0 (distance 2 mm). They show volume changes in the fusiform and lingual gyri as well as V5/MT+ bilaterally. In (B) the functional ROI (green) is superimposed; x, y, z, views at the two cluster maxima of V5/MT+ are shown.

Correlation of nystagmus severity with cerebellar volume in CN subjects

Cerebellar GM volume of the CN individuals correlated positively with nystagmus sway path (linear model) in vermis VIII (pyramis), IX (uvula), and X (nodulus; Table 3; Figure 2A). Correlations were also found with cerebellar hemisphere lobule II (left, lingula), hemisphere VI (right, lobus simplex), crus I (right, lobulus ansiformis), crus II (lobulus ansiformis), and lobule VII (lobus paramedianus), VIII (lobus biventer), and IX (paraflocculus) bilaterally. No negative correlations between cerebellar GM volume and nystagmus sway path were found in CN individuals, and no correlations with a positive or negative nystagmus angle were found (not shown). The normalized local cerebellar volume values were extracted at x = 6 y = −72 z = −48 (local cluster maximum) and plotted vs. nystagmus sway path (Figure 2B). VOI were extracted from several additional locations within the cerebellar clusters and all tested correlations were positive. For comparison, an analysis was also done for the group of controls by assuming their sway path to be 0. The variation of GM volume in the selected VOI was about the same in controls as in CN individuals (Figure 2B).

Table 3

Gray matter cerebellar volumes that correlated positively with CN sway path in CN subjects.

Anatomical location	MNI coordinates			Cluster size in voxels	T-value
	x	y	z
NYSTAGMUS SWAY PATH – POSITIVE CORRELATIONS
H VII R	6	−72	−48	2390	7.36
H VII L	−6	−72	−46		6.80
H VIII R	8	−68	−48		6.31
H IX R	6	−64	−48		5.29
H VIII L	−8	−66	−48		4.92
H IX L	−8	−61	−48		4.55
V VIII	−2	−65	−48		4.33
H Crus I R	39	−56	−34	239	6.89
H VI R	36	−55	−34		4.41
H IX L	−6	−48	−49	1041	5.40
V IX	−2	−51	−36		4.65
H IX R	4	−47	−47		4.40
V X	0	−48	−36		4.31
H II L	−29	−82	−49	69	4.91
H Crus II R	43	−77	−43	127	4.75
H Crus II L	−45	−75	−44	2	4.04

One-sample .

Figure 2

Results of correlation analysis of cerebellar gray matter volume with nystagmus severity in CN subjects. (A) A positive correlation of cerebellar gray matter volume with nystagmus sway path was found in CN subjects (linear model), while no overall changes in gray matter compared to controls were detected. In (B) the normalized local cerebellar volume values were plotted against nystagmus sway path for display purposes. Normalized local cerebellar volume values were extracted at x = 6 y = −72 z = −48 (local cluster maximum) within a specified volume of interest (VOI) to obtain an indirect measure of gray matter thickness (arbitrary units, AU). The triangles in (B) depict the wide variability of cerebellar volume in controls. In the CN subjects the linear correlation between the cerebellar volume and the nystagmus severity is demasked only by adding nystagmus sway path as a variable [circles in (B)]. The correlation statistics were calculated for the CN subjects at r = 0.85, p < 0.001. The r values must be interpreted with caution, since such values tend to be overestimated in this kind of analysis (Vul et al., 2009).

Discussion

Our aim in this study was to identify GM volume changes of the human brain that are associated with CN in patients without associated sensory deficits or metabolic disease. The main findings of our study were as follows: (1) the GM brain volume was larger in visually driven cortex areas including V5/MT+ in CN subjects than in controls. (2) There was a positive linear correlation between cerebellar GM volume in the vestibulo-cerebellum and adjacent vermal and paravermal structures with nystagmus sway path in subjects with CN. (3) No GM decreases were found in subjects with CN.

Gray matter volume increases in visually driven cortex areas and oscillopsia

The volume changes observed in the visually driven cortex areas in our subjects with CN were located within the lateral aspects of the anatomical landmarks described for V5/MT+ in studies (Dumoulin et al., 2000; Malikovic et al., 2007). There are two alternative explanations as to the functional significance of the GM volume increases observed in the current study. First, the mere viewing of a stationary scene activates V5/MT+ due to nystagmus and this excessive motion stimulation may cause the hypertrophy in CN. An fMRI study on albino subjects (all of whom had CN) investigated the decussation of optic nerve/tract fibers and found that static monocular checkerboard stimulation led to activation of the middle/inferior temporal gyrus in the CN subjects (Schmitz et al., 2004). This indicates that areas such as V5/MT+ can be activated due to nystagmus when static scenes are viewed. This interpretation is further supported by an fMRI study in patients with bilateral vestibular failure, who were stimulated with a visual motion paradigm. They had increased BOLD activations in V5/MT+ during optokinetic nystagmus compared to healthy controls (Dieterich et al., 2007). This was suggested to reflect increased visual sensitivity and interpreted as evidence of visual substitution for the vestibular failure. The amblyopia which was present in 8/14 CN subjects did not contribute to the GM increases in the visually driven cortex areas in our study. Additionally amblyopia in adults has been shown to be associated with GM volume decreases in visually driven cortex areas (Mendola et al., 2005). Alternatively, the volume increases could be due to the active suppression of oscillopsia in CN individuals. This is supported by psychophysical studies (Dieterich and Brandt, 1987). We also observed volume increases in the middle occipital gyrus and the fusiform gyrus bilaterally in CN subjects; both regions have been shown to be involved in the processing of motion stimuli (Shulman et al., 1998).

Cerebellar volume correlates with nystagmus severity

Intergroup comparison did not exhibit significant GM volume differences in the cerebellum. However, a significant correlation of various cerebellar structures could be demonstrated when the volume was correlated with nystagmus as measured by the sway path. A plausible explanation is provided by the large interindividual variability among CN individuals and healthy controls (Figure 2B): while the nystagmus sway path seems to drive the volume of cerebellar structures in CN subjects, other individual, or environmental as well as subject-specific factors must be responsible for the variations in cerebellar volume in controls. The GM volume correlations in the cerebellum can be separated roughly into vestibulo-cerebellar structures (vermis IX and X and hemispheres IX and X), structures adjacent to the vestibulo-cerebellum (vermis VIII and paravermal hemispheres lobules VII and VIII), and volume changes in the lateral cerebellar hemispheres (lobules II, VI, crus I, and crus II). The vermis and the vestibulo-cerebellum synergize during many ocular motor tasks; in CN they might act to suppress the excess eye movements. The role of the lateral hemispheric parts of the cerebellum during eye movements is much less clear. During CN they might be involved in generating them, as we will describe below.

Vermis VIII (pyramidis) and especially IX (uvula) and X (nodulus) are involved in smooth pursuit and optokinetic responses as has been shown in human (e.g., Baier et al., 2009) and non-human primates (e.g., Heinen and Keller, 1996). This has also been demonstrated for the paraflocculus/flocculus in animal experiments (Zee et al., 1981) as well as by functional imaging studies in humans (Kalla et al., 2006). Activations of the uvula, nodulus, and paraflocculus/flocculus (the vestibulo-cerebellum) have been described during fixation suppression of vestibular nystagmus in a PET study (Naito et al., 2003). The paraflocculus/flocculus is also important for gaze holding, because it exerts a stabilizing influence on brainstem integrators (Zee et al., 1981). We therefore interpret the hypertrophy of vestibulo-cerebellar and adjacent vermal and paravermal structures (hemispheres VII, VIII, and vermis VIII) in CN individuals to be the structural consequence of the constant attempt to inhibit a hyperoscillating system according to the model of Jacobs and Dell’Osso (2004).

Apart from the above-described vestibulo-cerebellar structures that most likely inhibit CN, there is also evidence that hypertrophy of hemisphere VI as well as crus I and II is associated with the generation of eye movements. In general, the role of the lateral cerebellar hemispheres for ocular motor control is more speculative: evidence comes from studies in humans with cerebellar lesions that impaired smooth pursuit initiation (Straube et al., 1997) as well as fMRI studies during optokinetic nystagmus (Bense et al., 2006). Using fMRI we were able to show activations of cerebellar hemispheres (including hemisphere VI and crus II) in individuals with CN while they looked in the direction of maximal nystagmus (unpublished observation). A similar observation was recently published suggesting that the cerebellar declive is involved in ocular motor dysfunction in idiopathic CN (Leguire et al., 2011). Volume increases in the cerebellar hemispheres were also observed in individuals with genetic syndromes, which can like other ophthalmologic and neurological abnormalities, be associated with CN. Additionally in these subjects the cerebellar vermis showed a GM volume loss that might lead to impaired central suppression of the CN in these individuals (Free et al., 2003).

Congenital and congenital-type nystagmus

There are a few single case descriptions of CN and congenital-type nystagmus which involve activation or alteration of cerebellar structures. A PET experiment showed a significant increase in the rCGM in the nodulus of a patient with pendular-type CN (Schlindwein et al., 2009). After an unsuccessful biopsy of a brainstem lesion, which resulted in the removal of a small piece of normal cerebellar tissue from the inferior uvula (IX) and adjacent pyramis (VIII), a horizontal congenital-type spontaneous nystagmus appeared with exponentially increasing velocity of the slow phases (Radtke et al., 2001). Similar nystagmus waveforms were observed in patients with Arnold-Chiari malformation or cerebellar degeneration (e.g., Nakahara and Murofushi, 2003).

Limitations of the study

Our study has several limitations which could potentially influence the interpretation of the results: first the fact that nystagmus severity was not measured during natural viewing conditions but during minimal and maximal nystagmus intensity. Second the finding that no decreases in GM were found in the CN subjects has to be viewed with caution since our analysis was not initially designed to allow for this kind of conclusion.

Conclusions

In conclusion, we found GM volume increases in subjects with CN. These increases can be related to different aspects of ocular motor and perceptual functions. It appears equally important that no distinct areas of GM volume decreases could be demonstrated. Further longitudinal studies are necessary to resolve whether a causative relationship exits between the observed GM brain volume changes and CN.

Conflict of Interest Statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.