Literature DB >> 2199327

Construction of broad-host-range vectors for the selection of divergent promoters.

S L Ronald1, A M Kropinski, M A Farinha.   

Abstract

A series of promoter-probe plasmid vectors has been constructed which allows for the selection of DNA sequences containing divergent control elements. Each vector contains a pair of promoterless genes [encoding beta-galactosidase (lacZ), alkaline phosphatase (phoA), and bacterial luciferase (luxAB)] arranged in an antiparallel fashion and separated by a large intervening multiple cloning site. The vectors permit direct detection of promoter activity on indicator plates after transformation. Cloned promoters are selected based on production of coloured products in the case of lacZ and phoA, and by the emission of light in the case of luxAB. These vectors have been tested using known divergent promoter elements from pBR322 and Pseudomonas phage D3.

Entities:  

Mesh：

Year:  1990        PMID： 2199327     DOI： 10.1016/0378-1119(90)90451-v

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  7 in total

1.  CT406 encodes a chlamydial ortholog of NrdR, a repressor of ribonucleotide reductase.

Authors:  Elizabeth Di Russo Case; Johnny C Akers; Ming Tan
Journal:  J Bacteriol       Date:  2011-07-01       Impact factor: 3.490

2.  Inactivation of gltB abolishes expression of the assimilatory nitrate reductase gene (nasB) in Pseudomonas putida KT2442.

Authors:  L Eberl; A Ammendola; M H Rothballer; M Givskov; C Sternberg; M Kilstrup; K H Schleifer; S Molin
Journal:  J Bacteriol       Date:  2000-06       Impact factor: 3.490

3.  A two-reporter gene system for the analysis of bi-directional transcription from the divergent MAL6T-MAL6S promoter in Saccharomyces cerevisiae.

Authors:  P J Bell; P H Bissinger; R J Evans; I W Dawes
Journal:  Curr Genet       Date:  1995-10       Impact factor: 3.886

4.  The sulfur-regulated arylsulfatase gene cluster of Pseudomonas aeruginosa, a new member of the cys regulon.

Authors:  J Hummerjohann; S Laudenbach; J Rétey; T Leisinger; M A Kertesz
Journal:  J Bacteriol       Date:  2000-04       Impact factor: 3.490

5.  Swarming-coupled expression of the Proteus mirabilis hpmBA haemolysin operon.

Authors:  Gillian M Fraser; Laurent Claret; Richard Furness; Srishti Gupta; Colin Hughes
Journal:  Microbiology       Date:  2002-07       Impact factor: 2.777

6.  Cloning of the early promoters of Pseudomonas aeruginosa bacteriophage D3: sequence of the immunity region of D3.

Authors:  M A Farinha; B J Allan; E M Gertman; S L Ronald; A M Kropinski
Journal:  J Bacteriol       Date:  1994-08       Impact factor: 3.490

7.  Visualizing the activity of Escherichia coli divergent promoters and probing their dependence on superhelical density using dual-colour fluorescent reporter vector.

Authors:  Irina S Masulis; Zaira Sh Babaeva; Sergey V Chernyshov; Olga N Ozoline
Journal:  Sci Rep       Date:  2015-06-17       Impact factor: 4.379
  7 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.