T helper 17 cells and regulatory T-cell imbalance in paediatric patients with asthma.

Asthma is a chronic inflammatory disorder of the lung, which is thought to be determined by the balance between the T helper (Th)2 and Th1 responses. This study evaluated whether the balance between Th17 cells and regulatory T cells (T(reg)) was impaired in asthma patients. The proportion of peripheral blood Th17 cells of the total CD4(+) cell population in asthma patients was significantly higher than in controls (mean ± SD 0.72 ± 0.5% versus 0.31 ± 0.4%, respectively). The proportion of peripheral T(reg) cells in asthma patients was significantly lower than in controls (mean ± SD 12.1 ± 4.6% versus 27.2 ± 7.5%, respectively). Analysis of mRNA generally confirmed the flow cytometry data, suggesting that the changes in cytokine levels were mediated at the transcription level. In paediatric asthma patients, the CD4(+) T-cell phenotype was skewed toward the Th17 phenotype, suggesting that a Th17/T(reg) functional imbalance plays a role in asthma.