Literature DB >> 21983412

Antibiotics-free stable polyhydroxyalkanoate (PHA) production from carbon dioxide by recombinant cyanobacteria.

Hideo Akiyama1, Hiroshi Okuhata, Takuo Onizuka, Shozo Kanai, Masahiko Hirano, Satoshi Tanaka, Ken Sasaki, Hitoshi Miyasaka.   

Abstract

A practical antibiotics-free plasmid expression system in cyanobacteria was developed by using the complementation of cyanobacterial recA null mutation with the EscherichiacolirecA gene on the plasmid. This system was applied to the production of polyhydroxyalkanoate (PHA), a biodegradable plastic, and the transgenic cyanobacteria stably maintained the pha genes for PHA production in the antibiotics-free medium, and accumulated up to 52% cell dry weight of PHA.
Copyright © 2011 Elsevier Ltd. All rights reserved.

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Year:  2011        PMID： 21983412     DOI： 10.1016/j.biortech.2011.09.058

Source DB:  PubMed          Journal:  Bioresour Technol        ISSN： 0960-8524            Impact factor:   9.642


  11 in total

Review 1.  Metabolic design for cyanobacterial chemical synthesis.

Authors:  John W K Oliver; Shota Atsumi
Journal:  Photosynth Res       Date:  2014-04-10       Impact factor: 3.573

2.  GC/MS-based 13C metabolic flux analysis resolves the parallel and cyclic photomixotrophic metabolism of Synechocystis sp. PCC 6803 and selected deletion mutants including the Entner-Doudoroff and phosphoketolase pathways.

Authors:  Dennis Schulze; Michael Kohlstedt; Judith Becker; Edern Cahoreau; Lindsay Peyriga; Alexander Makowka; Sarah Hildebrandt; Kirstin Gutekunst; Jean-Charles Portais; Christoph Wittmann
Journal:  Microb Cell Fact       Date:  2022-04-22       Impact factor: 6.352

3.  Efficient strategies to enhance plasmid stability for fermentation of recombinant Escherichia coli harboring tyrosine phenol lyase.

Authors:  Xiao-Ling Tang; Wen-Ye Hu; Zhi-Chao Wang; Ren-Chao Zheng; Yu-Guo Zheng
Journal:  Biotechnol Lett       Date:  2021-04-08       Impact factor: 2.461

Review 4.  Cyanobacteria: Photoautotrophic Microbial Factories for the Sustainable Synthesis of Industrial Products.

Authors:  Nyok-Sean Lau; Minami Matsui; Amirul Al-Ashraf Abdullah
Journal:  Biomed Res Int       Date:  2015-06-25       Impact factor: 3.411

5.  Draft Genome Sequence of the Picocyanobacterium Synechococcus sp. Strain GFB01, Isolated from a Freshwater Lagoon in the Brazilian Amazon.

Authors:  Pedro Ivo Guimarães; Tiago Ferreira Leão; Aline Grasielle Costa de Melo; Rommel Thiago Jucá Ramos; Artur Silva; Marli Fatima Fiore; Maria Paula Cruz Schneider
Journal:  Genome Announc       Date:  2015-08-13

Review 6.  Towards synthetic biological approaches to resource utilization on space missions.

Authors:  Amor A Menezes; John Cumbers; John A Hogan; Adam P Arkin
Journal:  J R Soc Interface       Date:  2015-01-06       Impact factor: 4.118

Review 7.  Synechocystis: Not Just a Plug-Bug for CO2, but a Green E. coli.

Authors:  Filipe Branco Dos Santos; Wei Du; Klaas J Hellingwerf
Journal:  Front Bioeng Biotechnol       Date:  2014-09-18

8.  Photoautotrophic production of polyhydroxyalkanoates in a synthetic mixed culture of Synechococcus elongatus cscB and Pseudomonas putida cscAB.

Authors:  Hannes Löwe; Karina Hobmeier; Manuel Moos; Andreas Kremling; Katharina Pflüger-Grau
Journal:  Biotechnol Biofuels       Date:  2017-07-19       Impact factor: 6.040

9.  Maximizing PHB content in Synechocystis sp. PCC 6803: a new metabolic engineering strategy based on the regulator PirC.

Authors:  Moritz Koch; Jonas Bruckmoser; Jörg Scholl; Waldemar Hauf; Bernhard Rieger; Karl Forchhammer
Journal:  Microb Cell Fact       Date:  2020-12-22       Impact factor: 5.328

10.  Construction of an Antibiotic-Free Vector and its Application in the Metabolic Engineering of Escherichia Coli for Polyhydroxybutyrate Production.

Authors:  Ying-Cheng Liao; Boonyawee Saengsawang; Jun-Wei Chen; Xiao-Zhen Zhuo; Si-Yu Li
Journal:  Front Bioeng Biotechnol       Date:  2022-06-03
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