BACKGROUND: An established C6 glioma was successfully treated with intratumoral injection of mesenchymal stem cells transduced with HSVtk gene (MSCtk) and systemic administration of ganciclovir (GCV). The best timing of GCV administration after the MSCtk implantation was studied. MATERIALS AND METHODS: GCV administration was started from 2 days before and 1, 3 and 7 days after the MSCtk administration under both in vitro and in vivo conditions. RESULTS: The C6 cells were completely eradicated in vitro when GCV administration was started from day -2, 1, and 3. Animals with intracranial tumor survived longer when GCV was administered earlier after MSCtk administration. This may, mainly, reflect the difference in the MSCtk/C6 ratio at the time of GCV administration because this ratio drastically decreases during the delay of GCV administration. CONCLUSION: When using a slowly growing vector cell as MSCtk, GCV should be administered soon after MSCtk implantation.
BACKGROUND: An established C6 glioma was successfully treated with intratumoral injection of mesenchymal stem cells transduced with HSVtk gene (MSCtk) and systemic administration of ganciclovir (GCV). The best timing of GCV administration after the MSCtk implantation was studied. MATERIALS AND METHODS:GCV administration was started from 2 days before and 1, 3 and 7 days after the MSCtk administration under both in vitro and in vivo conditions. RESULTS: The C6 cells were completely eradicated in vitro when GCV administration was started from day -2, 1, and 3. Animals with intracranial tumor survived longer when GCV was administered earlier after MSCtk administration. This may, mainly, reflect the difference in the MSCtk/C6 ratio at the time of GCV administration because this ratio drastically decreases during the delay of GCV administration. CONCLUSION: When using a slowly growing vector cell as MSCtk, GCV should be administered soon after MSCtk implantation.
Authors: Adam Nowakowski; Katarzyna Drela; Justyna Rozycka; Miroslaw Janowski; Barbara Lukomska Journal: Stem Cells Dev Date: 2016-09-07 Impact factor: 3.272
Authors: Antonella Mangraviti; Stephany Yi Tzeng; Kristen Lynn Kozielski; Yuan Wang; Yike Jin; David Gullotti; Mariangela Pedone; Nitsa Buaron; Ann Liu; David R Wilson; Sarah K Hansen; Fausto J Rodriguez; Guo-Dong Gao; Francesco DiMeco; Henry Brem; Alessandro Olivi; Betty Tyler; Jordan J Green Journal: ACS Nano Date: 2015-02-10 Impact factor: 15.881
Authors: Henry Ruiz-Garcia; Keila Alvarado-Estrada; Sunil Krishnan; Alfredo Quinones-Hinojosa; Daniel M Trifiletti Journal: Front Bioeng Biotechnol Date: 2020-12-07
Authors: Martin J Hicks; Maria J Chiuchiolo; Douglas Ballon; Jonathan P Dyke; Eric Aronowitz; Kosuke Funato; Viviane Tabar; David Havlicek; Fan Fan; Dolan Sondhi; Stephen M Kaminsky; Ronald G Crystal Journal: PLoS One Date: 2016-10-06 Impact factor: 3.240