Literature DB >> 21972073

An improved method for whole protein extraction from yeast Saccharomyces cerevisiae.

Tingting Zhang1, Jie Lei, Hanjiang Yang, Kun Xu, Rui Wang, Zhiying Zhang.   

Abstract

A new method for protein extraction from yeast Saccharomyces cerevisiae cells is described. This method involves the use of LiAc and NaOH to enhance the permeability of yeast cell wall prior to protein extraction with SDS-PAGE sample buffer. It was safe and efficient compared to other methods reported so far in the literature. The proteins extracted with this new method retained their immunoreactive properties and are suitable for most applications in molecular biology studies.
Copyright © 2011 John Wiley & Sons, Ltd.

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Year:  2011        PMID： 21972073     DOI： 10.1002/yea.1905

Source DB:  PubMed          Journal:  Yeast        ISSN： 0749-503X            Impact factor:   3.239


  69 in total

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