Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Expression and characterization of Cryptococcus neoformans recombinant App1.

Literature DB >> 21971702

Expression and characterization of Cryptococcus neoformans recombinant App1.

Asfia Qureshi¹, Virginia Williams, Maurizio Del Poeta.

Abstract

We characterized Cryptococcus neoformans recombinant antiphagocytic protein 1 (rApp1) by SDS-PAGE, gel filtration chromatography, circular dichroism, and fluorescence spectroscopy. rApp1 produced by C. neoformans var. grubii contains an odd number of cysteines; therefore, it has the ability to form intermolecular disulfide bridges which can lead to the formation of amyloid fibrils in the absence of β-mercaptoethanol or DTT in vitro. Alternate approaches to over-expression of rApp1 in the Lepidopteran High Five(™) Insect cell line using pIZ/V5-His and in lentivirus were explored and are described. Finally, localization of App1 in vivo was examined in the presence and absence of the capsule.

Entities: CellLine Chemical Disease Gene Species

Mesh：

Substances：

Year: 2011 PMID： 21971702 PMCID： PMC5125079 DOI： 10.1007/s11046-011-9486-7

Source DB: PubMed Journal: Mycopathologia ISSN： 0301-486X Impact factor: 2.574

15 in total

1. Formation of amyloid fibrils by peptides derived from the bacterial cold shock protein CspB.

Authors: M Gross; D K Wilkins; M C Pitkeathly; E W Chung; C Higham; A Clark; C M Dobson
Journal: Protein Sci Date: 1999-06 Impact factor: 6.725

Review 2. The contribution of melanin to microbial pathogenesis.

Authors: Joshua D Nosanchuk; Arturo Casadevall
Journal: Cell Microbiol Date: 2003-04 Impact factor: 3.715

3. Detection and prevention of protein aggregation before, during, and after purification.

Authors: Sarah E Bondos; Alicia Bicknell
Journal: Anal Biochem Date: 2003-05-15 Impact factor: 3.365

4. Imaging linear birefringence and dichroism in cerebral amyloid pathologies.

Authors: Lee-Way Jin; Kacey A Claborn; Miki Kurimoto; Morten A Geday; Izumi Maezawa; Faranak Sohraby; Marcus Estrada; Werner Kaminksy; Bart Kahr
Journal: Proc Natl Acad Sci U S A Date: 2003-12-10 Impact factor: 11.205

Review 5. Techniques to study amyloid fibril formation in vitro.

Authors: Melanie R Nilsson
Journal: Methods Date: 2004-09 Impact factor: 3.608

Review 6. Impact of melanin on microbial virulence and clinical resistance to antimicrobial compounds.

Authors: Joshua D Nosanchuk; Arturo Casadevall
Journal: Antimicrob Agents Chemother Date: 2006-11 Impact factor: 5.191

7. The physical properties of the capsular polysaccharides from Cryptococcus neoformans suggest features for capsule construction.

Authors: Diane C McFadden; Magdia De Jesus; Arturo Casadevall
Journal: J Biol Chem Date: 2005-11-08 Impact factor: 5.157

Expression and characterization of Cryptococcus neoformans recombinant App1.

1. Formation of amyloid fibrils by peptides derived from the bacterial cold shock protein CspB.

Review 2. The contribution of melanin to microbial pathogenesis.

3. Detection and prevention of protein aggregation before, during, and after purification.

4. Imaging linear birefringence and dichroism in cerebral amyloid pathologies.

Review 5. Techniques to study amyloid fibril formation in vitro.

Review 6. Impact of melanin on microbial virulence and clinical resistance to antimicrobial compounds.

7. The physical properties of the capsular polysaccharides from Cryptococcus neoformans suggest features for capsule construction.

8. Role of glucose in the expression of Cryptococcus neoformans antiphagocytic protein 1, App1.

9. Estimation of globular protein secondary structure from circular dichroism.

10. Melanin as a potential cryptococcal defence against microbicidal proteins.

1. Cryptococcus and cryptococcosis in the twenty-first century.

2. Cell Wall-Associated Virulence Factors Contribute to Increased Resilience of Old Cryptococcus neoformans Cells.