PURPOSE: Development of an improved animal model for studying skin burns in rats. METHODS: Twenty-four male Wistar rats were randomly assigned to four groups (n=6): G1-Control, G2- T100°C, G3-T150°C and G4-T200°C. Two 10 x 10 mm squares were outlined with a sterile surgical marker on each side and along the vertebral column using a prepared template positioned between the anterior and posterior limbs. G2-G4 rats were subjected to 100°C, 150°C and 200ºC thermal burns, respectively. G1 rats served as controls. Burns were inflicted by applying a copper plate connected to an electronic temperature controlling device to the dorsal skin of anesthetized rats. Four burns were produced on each animal (total area: 4 cm²/animal) leaving about 1 cm of undamaged skin between burn areas. Analgesia was administered during 24 h after burn injury by adding 30 mg codeine phosphate hemihydrate to 500 ml tap water. RESULTS: The application of 100°C and 150ºC resulted in partial thickness skin burns with central reepithelialization of the burned area only at 100°C. In G4 group the whole thickness of the skin was injured without central reepithelialization. However, there was marginal reepithelialization in all groups. CONCLUSION: The model studied is inexpensive and easily reproducible, enabling the achievement of controlled burns with partial or total impairment of the skin in experimental animals.
PURPOSE: Development of an improved animal model for studying skin burns in rats. METHODS: Twenty-four male Wistar rats were randomly assigned to four groups (n=6): G1-Control, G2- T100°C, G3-T150°C and G4-T200°C. Two 10 x 10 mm squares were outlined with a sterile surgical marker on each side and along the vertebral column using a prepared template positioned between the anterior and posterior limbs. G2-G4 rats were subjected to 100°C, 150°C and 200ºC thermal burns, respectively. G1 rats served as controls. Burns were inflicted by applying a copper plate connected to an electronic temperature controlling device to the dorsal skin of anesthetized rats. Four burns were produced on each animal (total area: 4 cm²/animal) leaving about 1 cm of undamaged skin between burn areas. Analgesia was administered during 24 h after burn injury by adding 30 mg codeine phosphate hemihydrate to 500 ml tap water. RESULTS: The application of 100°C and 150ºC resulted in partial thickness skin burns with central reepithelialization of the burned area only at 100°C. In G4 group the whole thickness of the skin was injured without central reepithelialization. However, there was marginal reepithelialization in all groups. CONCLUSION: The model studied is inexpensive and easily reproducible, enabling the achievement of controlled burns with partial or total impairment of the skin in experimental animals.
Authors: Moises Oliveira Dos Santos; Anne Latrive; Pedro Arthur Augusto De Castro; Wagner De Rossi; Telma Maria Tenorio Zorn; Ricardo Elgul Samad; Anderson Zanardi Freitas; Carlos Lenz Cesar; Nilson Dias Vieira Junior; Denise Maria Zezell Journal: Biomed Opt Express Date: 2017-02-16 Impact factor: 3.732
Authors: Danielle dos Santos Tavares Pereira; Maria Helena Madruga Lima-Ribeiro; Nicodemos Teles de Pontes-Filho; Ana Maria dos Anjos Carneiro-Leão; Maria Tereza dos Santos Correia Journal: J Biomed Biotechnol Date: 2012-03-29
Authors: Siiri E Iismaa; Xenia Kaidonis; Amy M Nicks; Nikolay Bogush; Kazu Kikuchi; Nawazish Naqvi; Richard P Harvey; Ahsan Husain; Robert M Graham Journal: NPJ Regen Med Date: 2018-02-23
Authors: Vlad Porumb; Alexandru Florentin Trandabăț; Cristina Terinte; Irina Draga Căruntu; Elena Porumb-Andrese; Mihail Gabriel Dimofte; Dragoş Pieptu Journal: Biomed Res Int Date: 2017-10-12 Impact factor: 3.411