BACKGROUND: Mycoplasma hominis, a human urogenital pathogen, is involved in genital and extragenital infections and arthritis, particularly in immunocompromised patients. The interleukin (IL) 23/T helper (Th) 17 axis is associated with inflammatory and autoimmune diseases. The aim of this study was to assess the IL-23 response to M. hominis in human dendritic cells (DCs) and the CD4(+) T-cell differentiation in response to M. hominis-infected DCs. METHODS: Human monocyte-derived DCs were cultured with phosphate-buffered saline, lipopolysaccharide, or M. hominis PG21. Cocultures with heterologous T cells were performed. Extracts from M. hominis were separated and incubated with DCs. Isolates from different clinical syndromes were tested. RESULTS: M. hominis induced the maturation of human DCs with predominant IL-23 secretion in a Toll-like receptor 2-dependent manner. The in vitro immunomodulatory capacity of M. hominis was contained in a lipoprotein-enriched fraction from the mycoplasma. M. hominis-activated DCs induced IL-17-producing CD4(+) T cells. Interestingly, clinical isolates differed in their ability to promote IL-23 secretion by DCs. CONCLUSIONS: Taken together, our findings demonstrate a major role for the IL-23/Th17 axis in the defense against M. hominis and indicate a potential role for these bacteria in inflammatory and autoimmune diseases.
BACKGROUND:Mycoplasma hominis, a human urogenital pathogen, is involved in genital and extragenital infections and arthritis, particularly in immunocompromised patients. The interleukin (IL) 23/T helper (Th) 17 axis is associated with inflammatory and autoimmune diseases. The aim of this study was to assess the IL-23 response to M. hominis in human dendritic cells (DCs) and the CD4(+) T-cell differentiation in response to M. hominis-infected DCs. METHODS:Human monocyte-derived DCs were cultured with phosphate-buffered saline, lipopolysaccharide, or M. hominis PG21. Cocultures with heterologous T cells were performed. Extracts from M. hominis were separated and incubated with DCs. Isolates from different clinical syndromes were tested. RESULTS:M. hominis induced the maturation of human DCs with predominant IL-23 secretion in a Toll-like receptor 2-dependent manner. The in vitro immunomodulatory capacity of M. hominis was contained in a lipoprotein-enriched fraction from the mycoplasma. M. hominis-activated DCs induced IL-17-producing CD4(+) T cells. Interestingly, clinical isolates differed in their ability to promote IL-23 secretion by DCs. CONCLUSIONS: Taken together, our findings demonstrate a major role for the IL-23/Th17 axis in the defense against M. hominis and indicate a potential role for these bacteria in inflammatory and autoimmune diseases.
Authors: J Goret; L Béven; B Faustin; C Contin-Bordes; C Le Roy; S Claverol; H Renaudin; C Bébéar; S Pereyre Journal: J Bacteriol Date: 2017-07-11 Impact factor: 3.490
Authors: Thamotharampillai Dileepan; Erica D Smith; Daniel Knowland; Martin Hsu; Maryann Platt; Peter Bittner-Eddy; Brenda Cohen; Peter Southern; Elizabeth Latimer; Earl Harley; Dritan Agalliu; P Patrick Cleary Journal: J Clin Invest Date: 2015-12-14 Impact factor: 14.808
Authors: Hannah E Jones; Alastair Copland; Hendrik Jan Hamstra; Jonathan Cohen; Jeremy Brown; Nigel Klein; Peter van der Ley; Garth Dixon Journal: Cell Microbiol Date: 2013-11-06 Impact factor: 3.715
Authors: Aleksandra Sobolewska; Marcin Włodarczyk; Krystyna Stec-Michalska; Jakub Fichna; Maria Wiśniewska-Jarosińska Journal: Dig Dis Sci Date: 2015-10-06 Impact factor: 3.199