OBJECTIVE: To identify the optimal method of submission of canine and feline urine for bacterial culture. METHODS: Cystocentesis samples from 250 animals (200 dogs, 50 cats) suspected of having urinary tract infections were collected. The reference aliquot, without preservative, was processed on site within 2 hours. Two further aliquots (one without preservative, one with boric acid) were stored at room temperature for up to 7 hours and then posted by guaranteed next day delivery to a commercial laboratory for analysis. RESULTS: Forty-seven of the samples were positive on culture in the reference test. There was no significant difference between reference test results and those of samples posted without preservative (P=0·39), but samples posted in boric acid were significantly less likely to give a positive result (P=0·01). Samples posted without preservative had a sensitivity of 82% and a specificity of 98%; for boric acid, sensitivity was 73% and specificity 99%. CLINICAL SIGNIFICANCE: Postal urine samples should be submitted to the laboratory in a plain sterile tube.
OBJECTIVE: To identify the optimal method of submission of canine and feline urine for bacterial culture. METHODS: Cystocentesis samples from 250 animals (200 dogs, 50 cats) suspected of having urinary tract infections were collected. The reference aliquot, without preservative, was processed on site within 2 hours. Two further aliquots (one without preservative, one with boric acid) were stored at room temperature for up to 7 hours and then posted by guaranteed next day delivery to a commercial laboratory for analysis. RESULTS: Forty-seven of the samples were positive on culture in the reference test. There was no significant difference between reference test results and those of samples posted without preservative (P=0·39), but samples posted in boric acid were significantly less likely to give a positive result (P=0·01). Samples posted without preservative had a sensitivity of 82% and a specificity of 98%; for boric acid, sensitivity was 73% and specificity 99%. CLINICAL SIGNIFICANCE: Postal urine samples should be submitted to the laboratory in a plain sterile tube.
Authors: Hui Li; Peter Torab; Kathleen E Mach; Christine Surrette; Matthew R England; David W Craft; Neal J Thomas; Joseph C Liao; Chris Puleo; Pak Kin Wong Journal: Proc Natl Acad Sci U S A Date: 2019-05-08 Impact factor: 11.205
Authors: T M Sørensen; C R Bjørnvad; G Cordoba; P Damborg; L Guardabassi; V Siersma; L Bjerrum; L R Jessen Journal: J Vet Intern Med Date: 2018-02-22 Impact factor: 3.333