OBJECTIVES: To assess the predictive value of in vitro drug susceptibility testing (DST) in slow-growing non-tuberculous mycobacteria (NTM), knowledge on quantitative levels of drug susceptibility should be available. The aim of this study was to investigate the suitability of the MGIT 960/TB eXiST system for quantitative DST of NTM. METHODS: We have assessed quantitative levels of drug susceptibility for clinical isolates of Mycobacterium avium, Mycobacterium intracellulare and Mycobacterium kansasii by comparing radiometric Bactec 460TB-based DST with non-radiometric DST using MGIT 960/TB eXiST. RESULTS: MGIT 960/TB eXiST gives results comparable to those of Bactec 460TB. CONCLUSIONS: The MGIT 960/TB eXiST appears suitable for quantitative DST of NTM.
OBJECTIVES: To assess the predictive value of in vitro drug susceptibility testing (DST) in slow-growing non-tuberculous mycobacteria (NTM), knowledge on quantitative levels of drug susceptibility should be available. The aim of this study was to investigate the suitability of the MGIT 960/TB eXiST system for quantitative DST of NTM. METHODS: We have assessed quantitative levels of drug susceptibility for clinical isolates of Mycobacterium avium, Mycobacterium intracellulare and Mycobacterium kansasii by comparing radiometric Bactec 460TB-based DST with non-radiometric DST using MGIT 960/TB eXiST. RESULTS: MGIT 960/TB eXiST gives results comparable to those of Bactec 460TB. CONCLUSIONS: The MGIT 960/TB eXiST appears suitable for quantitative DST of NTM.
Authors: Pieter-Jan Ceyssens; Karine Soetaert; Markus Timke; An Van den Bossche; Katrin Sparbier; Koen De Cremer; Markus Kostrzewa; Marijke Hendrickx; Vanessa Mathys Journal: J Clin Microbiol Date: 2016-12-21 Impact factor: 5.948