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Literature DB >> 21964784

Profiling cellular myristoylation and palmitoylation using ω-alkynyl fatty acids.

Abstract

Methods to detect and characterize cellular protein myristoylation and palmitoylation are invaluable in cell biology, immunology, and virology. Recently, we developed ω-alkynyl fatty acid probes for monitoring myristoylation and palmitoylation of cellular proteins. This article describes a biochemical procedure for metabolic labeling of cells with ω-alkynyl fatty acids and click chemistry.

Entities: Chemical

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Year: 2012 PMID： 21964784 DOI： 10.1007/978-1-61779-349-3_7

Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745

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Cited

10 in total

1. Single-cell in situ imaging of palmitoylation in fatty-acylated proteins.

Authors: Xinxin Gao; Rami N Hannoush
Journal: Nat Protoc Date: 2014-10-09 Impact factor: 13.491

2. Fluorescent imaging of protein myristoylation during cellular differentiation and development.

Authors: Andrew J Witten; Karin F K Ejendal; Lindsey M Gengelbach; Meghan A Traore; Xu Wang; David M Umulis; Sarah Calve; Tamara L Kinzer-Ursem
Journal: J Lipid Res Date: 2017-07-28 Impact factor: 5.922

3. Notum is required for neural and head induction via Wnt deacylation, oxidation, and inactivation.

Authors: Xinjun Zhang; Seong-Moon Cheong; Nathalia G Amado; Alice H Reis; Bryan T MacDonald; Matthias Zebisch; E Yvonne Jones; Jose Garcia Abreu; Xi He
Journal: Dev Cell Date: 2015-03-12 Impact factor: 12.270

4. Bioorthogonal click chemistry to assay mu-opioid receptor palmitoylation using 15-hexadecynoic acid and immunoprecipitation.

Authors: Brittany Ebersole; Jessica Petko; Robert Levenson
Journal: Anal Biochem Date: 2014-01-23 Impact factor: 3.365

Profiling cellular myristoylation and palmitoylation using ω-alkynyl fatty acids.

1. Single-cell in situ imaging of palmitoylation in fatty-acylated proteins.

2. Fluorescent imaging of protein myristoylation during cellular differentiation and development.

3. Notum is required for neural and head induction via Wnt deacylation, oxidation, and inactivation.

4. Bioorthogonal click chemistry to assay mu-opioid receptor palmitoylation using 15-hexadecynoic acid and immunoprecipitation.

Review 5. The physiology of protein S-acylation.

Review 6. A Not-So-Ancient Grease History: Click Chemistry and Protein Lipid Modifications.

7. Palmitoylation of gephyrin controls receptor clustering and plasticity of GABAergic synapses.

8. Autopalmitoylation of TEAD proteins regulates transcriptional output of the Hippo pathway.

Review 9. Ion channel regulation by protein S-acylation.

10. Detecting N-myristoylation and S-acylation of host and pathogen proteins in plants using click chemistry.