OBJECTIVE: We aimed to measure separately the contributions of heat and humidity to changes in levels of 34 markers of inborn disorders in dried-blood-spot (DBS) samples. DESIGN AND METHODS: We stored paired sets of DBSs at 37°C for predetermined intervals in low-humidity and high-humidity environments. Marker levels of all samples in each complete sample set were measured in a single analytic run. RESULTS: During the 30 ± 5 day studies, galactose-1-phosphate uridyltransferase and biotinidase lost almost 65% of initial activities in low-humidity storage; most of the degradation in 27 other markers was attributable to adverse effects of high-humidity storage; seven markers in DBSs stored at high humidity lost more than 90% of initial levels by the end of the study and 4 of the 7 lost more than 50% of initial levels within the first week of storage. CONCLUSIONS: Minimizing both humidity and temperature in DBS transportation and storage environments is essential to maintaining sample integrity.
OBJECTIVE: We aimed to measure separately the contributions of heat and humidity to changes in levels of 34 markers of inborn disorders in dried-blood-spot (DBS) samples. DESIGN AND METHODS: We stored paired sets of DBSs at 37°C for predetermined intervals in low-humidity and high-humidity environments. Marker levels of all samples in each complete sample set were measured in a single analytic run. RESULTS: During the 30 ± 5 day studies, galactose-1-phosphate uridyltransferase and biotinidase lost almost 65% of initial activities in low-humidity storage; most of the degradation in 27 other markers was attributable to adverse effects of high-humidity storage; seven markers in DBSs stored at high humidity lost more than 90% of initial levels by the end of the study and 4 of the 7 lost more than 50% of initial levels within the first week of storage. CONCLUSIONS: Minimizing both humidity and temperature in DBS transportation and storage environments is essential to maintaining sample integrity.
Authors: B L Therrell; W H Hannon; K A Pass; F Lorey; C Brokopp; J Eckman; M Glass; R Heidenreich; S Kinney; S Kling; G Landenburger; F J Meaney; E R McCabe; S Panny; M Schwartz; E Shapira Journal: Biochem Mol Med Date: 1996-04
Authors: Donald H Chace; Timothy Lim; Christina R Hansen; Victor R De Jesus; W Harry Hannon Journal: Clin Chim Acta Date: 2009-06-21 Impact factor: 3.786
Authors: Jonathan A Kluge; Adrian B Li; Brooke T Kahn; Dominique S Michaud; Fiorenzo G Omenetto; David L Kaplan Journal: Proc Natl Acad Sci U S A Date: 2016-05-09 Impact factor: 11.205