BACKGROUND & AIMS: Population studies indicate that greater red meat consumption increases colorectal cancer risk while dietary fibre is protective. Previous work in rats showed that diets high in protein, including red meat, increase colonocyte DNA strand breaks and that this effect is attenuated by resistant starches (RS). Telomeres are long hexamer repeats that protect against spontaneous DNA damage which would lead to chromosomal instability. Telomere shortening is associated with greater risk of colorectal cancer. The current study aimed to determine the effects of cooked red and white meat intake on colonocyte telomere length in rats and whether dietary RS modified their effects. METHODS: After four weeks of feeding cooked beef or chicken at 15, 25 and 35% of diet with or without RS, colonocyte telomere length was measured. RESULTS: Telomere length decreased in proportion to red meat content of the diet. A similar trend was observed in the white meat group. Colonocyte telomere shortening due to increased dietary meat was attenuated by the inclusion of RS. CONCLUSION: These data support previous findings of increased colonocyte DNA damage with greater red and white meat intake and also the protective effect of dietary fibre. Crown
BACKGROUND & AIMS: Population studies indicate that greater red meat consumption increases colorectal cancer risk while dietary fibre is protective. Previous work in rats showed that diets high in protein, including red meat, increase colonocyte DNA strand breaks and that this effect is attenuated by resistant starches (RS). Telomeres are long hexamer repeats that protect against spontaneous DNA damage which would lead to chromosomal instability. Telomere shortening is associated with greater risk of colorectal cancer. The current study aimed to determine the effects of cooked red and white meat intake on colonocyte telomere length in rats and whether dietary RS modified their effects. METHODS: After four weeks of feeding cooked beef or chicken at 15, 25 and 35% of diet with or without RS, colonocyte telomere length was measured. RESULTS: Telomere length decreased in proportion to red meat content of the diet. A similar trend was observed in the white meat group. Colonocyte telomere shortening due to increased dietary meat was attenuated by the inclusion of RS. CONCLUSION: These data support previous findings of increased colonocyte DNA damage with greater red and white meat intake and also the protective effect of dietary fibre. Crown
Authors: David C Dallas; Megan R Sanctuary; Yunyao Qu; Shabnam Haghighat Khajavi; Alexandria E Van Zandt; Melissa Dyandra; Steven A Frese; Daniela Barile; J Bruce German Journal: Crit Rev Food Sci Nutr Date: 2017-10-13 Impact factor: 11.176
Authors: Richard K Le Leu; Jean M Winter; Claus T Christophersen; Graeme P Young; Karen J Humphreys; Ying Hu; Silvia W Gratz; Rosalind B Miller; David L Topping; Anthony R Bird; Michael A Conlon Journal: Br J Nutr Date: 2015-06-17 Impact factor: 3.718
Authors: Kim Y C Fung; Cheng Cheng Ooi; Michelle H Zucker; Trevor Lockett; Desmond B Williams; Leah J Cosgrove; David L Topping Journal: Int J Mol Sci Date: 2013-06-27 Impact factor: 5.923