| Literature DB >> 21959040 |
Tatsuro Naganuma1, Yuichiro Sato, Takayuki Sassa, Yusuke Ohno, Akio Kihara.
Abstract
Very long-chain fatty acids (VLCFAs) have a variety of physiological functions and are related to numerous disorders. The key step of VLCFA elongation is catalyzed by members of the elongase family, ELOVLs. Mammals have seven ELOVLs (ELOVL1-7), yet none of them has been purified and analyzed. In the presented study we purified ELOVL7 and measured its activity by reconstituting it into proteoliposomes. Purified ELOVL7 exhibited high activity toward acyl-CoAs with C18 carbon chain length. The calculated K(m) values toward C18:3(n-3)-CoA and malonyl-CoA were both in the μM range. We also found that progression of the VLCFA cycle enhances ELOVL7 activity.Entities:
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Year: 2011 PMID： 21959040 DOI： 10.1016/j.febslet.2011.09.024
Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124