| Literature DB >> 21957198 |
Theodoros Kelesidis1, Judith S Currier2, Diana Huynh2, David Meriwether3, Christina Charles-Schoeman2, Srinivasa T Reddy4, Alan M Fogelman2, Mohamad Navab2, Otto O Yang5.
Abstract
Most current assays of HDL functional properties are cell-based. We have developed a fluorometric biochemical assay based on the oxidation of dihydrorhodamine 123 (DHR) by HDL. This cell-free assay assesses the intrinsic ability of HDL to be oxidized by measuring increasing fluorescence due to DHR oxidation over time. The assay distinguishes the oxidative potential of HDL taken from different persons, and the results are reproducible. Direct comparison of this measurement correlated well with results obtained using a validated cell-based assay (r(2) = 0.62, P < 0.001). The assay can be scaled from a 96-well format to a 384-well format and, therefore, is suitable for high-throughput implementation. This new fluorometric method offers an inexpensive, accurate, and rapid means for determining the oxidative properties of HDL that is applicable to large-scale clinical studies.Entities:
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Year: 2011 PMID： 21957198 PMCID： PMC3220300 DOI： 10.1194/jlr.D018937
Source DB: PubMed Journal: J Lipid Res ISSN： 0022-2275 Impact factor: 5.922