Literature DB >> 2195042

Nuclear and cytoplasmic localization of different basic fibroblast growth factor species.

M Renko1, N Quarto, T Morimoto, D B Rifkin.   

Abstract

The subcellular distribution of basic fibroblastic growth factor (bFGF) was analyzed by subcellular fractionation and immunofluorescence to gain insight into potential mechanisms for its release from cells. Subcellular fractionation of either SK-Hep-1 cells or NIH 3T3 cells transfected with a bFGF cDNA revealed that the 18 kd form of bFGF was found primarily in the cytosolic fraction, whereas the 22 and 24 kd forms of bFGF were found preferentially in ribosomal and nuclear fractions. Analysis of bFGF distribution by immunofluorescence using an antibody that recognized all forms of bFGF indicated both cytoplasmic and nuclear localization but failed to reveal any growth factor in structures representing secretory vesicles. Therefore, bFGF has a distribution inconsistent with that of a secretory protein.

Entities:  

Mesh：

Substances：

Year:  1990        PMID： 2195042     DOI： 10.1002/jcp.1041440114

Source DB:  PubMed          Journal:  J Cell Physiol        ISSN： 0021-9541            Impact factor:   6.384


  53 in total

1.  Nuclear activities of basic fibroblast growth factor: potentiation of low-serum growth mediated by natural or chimeric nuclear localization signals.

Authors:  M Arese; Y Chen; R Z Florkiewicz; A Gualandris; B Shen; D B Rifkin
Journal:  Mol Biol Cell       Date:  1999-05       Impact factor: 4.138

2.  Ultrastructural immunolocalization of basic fibroblast growth factor in endothelial cells: morphologic evidence for unconventional secretion of a novel protein.

Authors:  Ranan Gulhan Aktas; Robert J Kayton
Journal:  J Mol Histol       Date:  2011-08-10       Impact factor: 2.611

3.  Expression of acidic and basic fibroblast growth factors in the substantia nigra of rat, monkey, and human.

Authors:  A J Bean; R Elde; Y H Cao; C Oellig; C Tamminga; M Goldstein; R F Pettersson; T Hökfelt
Journal:  Proc Natl Acad Sci U S A       Date:  1991-11-15       Impact factor: 11.205

4.  Basic fibroblastic growth factor as a potential meningeal angiogenic factor.

Authors:  J J Olson; A Reisner; J M Klemm; R A Bakay
Journal:  Skull Base Surg       Date:  1993

5.  Human dermal fibroblasts express multiple bFGF and aFGF proteins.

Authors:  L L Root; G D Shipley
Journal:  In Vitro Cell Dev Biol       Date:  1991-10

6.  Molecular atherectomy for restenosis.

Authors:  W Casscells; D A Lappi; A Baird
Journal:  Trends Cardiovasc Med       Date:  1993 Nov-Dec       Impact factor: 6.677

7.  Exon switching and activation of stromal and embryonic fibroblast growth factor (FGF)-FGF receptor genes in prostate epithelial cells accompany stromal independence and malignancy.

Authors:  G Yan; Y Fukabori; G McBride; S Nikolaropolous; W L McKeehan
Journal:  Mol Cell Biol       Date:  1993-08       Impact factor: 4.272

Review 8.  High molecular weight FGF2: the biology of a nuclear growth factor.

Authors:  K Chlebova; V Bryja; P Dvorak; A Kozubik; W R Wilcox; P Krejci
Journal:  Cell Mol Life Sci       Date:  2009-01       Impact factor: 9.261

9.  Translocation of FGF-1 and FGF-2 across vesicular membranes occurs during G1-phase by a common mechanism.

Authors:  Jedrzej Małecki; Jørgen Wesche; Camilla Skiple Skjerpen; Antoni Wiedłocha; Sjur Olsnes
Journal:  Mol Biol Cell       Date:  2003-12-02       Impact factor: 4.138

10.  Increase of basic fibroblast growth factor immunoreactivity and its mRNA level in rat brain following transient forebrain ischemia.

Authors:  K Takami; M Iwane; Y Kiyota; M Miyamoto; R Tsukuda; S Shiosaka
Journal:  Exp Brain Res       Date:  1992       Impact factor: 1.972
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.