OBJECTIVE: Vascular endothelial growth factors (VEGFs) are involved in angiogenesis, but molecular links to the most important etiological agents, human papillomavirus (HPV) and smoking, need to be clarified. METHODS: Archival samples at the first diagnosis of 64 cervical intraepithelial neoplasia grade 1 or 2 (CIN 1/2) lesions were examined immunohistochemically using anti-VEGF-C and anti-Ki-67 antibodies. HPV types were identified from cervical samples by restriction fragment length polymorphism, which has been shown to identify at least 26 types of genital HPVs. Follow-up data were available for all patients with CIN lesions. RESULTS: Cervical intraepithelial neoplasia lesions regressed in 47 cases and were persistent in 17 cases. Twenty-two smokers, 8 former smokers, and 34 non-smokers were enrolled in the study. The median observation period was 52.3 months. Significantly higher VEGF-C expression was observed in 8 smokers with persistent CIN persistence (49.0 ± 16.6%, P < 0.01), whereas no significant difference was observed in Ki-67 expression. The median time to regression was significantly longer in the 10 smokers with high VEGF-C expression (48.3 months, P = 0.030) than that in the others. HPV was detected in 56 of the 64 cases. Thirty-two patients had high-risk HPV, 13 had intermediate-risk HPV, and 2 had low-risk HPV. No significant difference was observed among the HPV risk groups in both average Ki-67 and VEGF-C expression. CONCLUSIONS: These findings suggest that VEGF-C may play an important role in cigarette smoking-associated cervical carcinogenesis.
OBJECTIVE: Vascular endothelial growth factors (VEGFs) are involved in angiogenesis, but molecular links to the most important etiological agents, human papillomavirus (HPV) and smoking, need to be clarified. METHODS: Archival samples at the first diagnosis of 64 cervical intraepithelial neoplasia grade 1 or 2 (CIN 1/2) lesions were examined immunohistochemically using anti-VEGF-C and anti-Ki-67 antibodies. HPV types were identified from cervical samples by restriction fragment length polymorphism, which has been shown to identify at least 26 types of genital HPVs. Follow-up data were available for all patients with CIN lesions. RESULTS:Cervical intraepithelial neoplasia lesions regressed in 47 cases and were persistent in 17 cases. Twenty-two smokers, 8 former smokers, and 34 non-smokers were enrolled in the study. The median observation period was 52.3 months. Significantly higher VEGF-C expression was observed in 8 smokers with persistent CIN persistence (49.0 ± 16.6%, P < 0.01), whereas no significant difference was observed in Ki-67 expression. The median time to regression was significantly longer in the 10 smokers with high VEGF-C expression (48.3 months, P = 0.030) than that in the others. HPV was detected in 56 of the 64 cases. Thirty-two patients had high-risk HPV, 13 had intermediate-risk HPV, and 2 had low-risk HPV. No significant difference was observed among the HPV risk groups in both average Ki-67 and VEGF-C expression. CONCLUSIONS: These findings suggest that VEGF-C may play an important role in cigarette smoking-associated cervical carcinogenesis.
Authors: H Nagano; H Yoshikawa; T Kawana; H Yokota; Y Taketani; H Igarashi; H Yoshikura; A Iwamoto Journal: J Obstet Gynaecol Res Date: 1996-02 Impact factor: 1.730
Authors: S J Mandriota; L Jussila; M Jeltsch; A Compagni; D Baetens; R Prevo; S Banerji; J Huarte; R Montesano; D G Jackson; L Orci; K Alitalo; G Christofori; M S Pepper Journal: EMBO J Date: 2001-02-15 Impact factor: 11.598
Authors: Cornelia L Trimble; Jeanine M Genkinger; Alyce E Burke; Sandra C Hoffman; Kathy J Helzlsouer; Marie Diener-West; George W Comstock; Anthony J Alberg Journal: Obstet Gynecol Date: 2005-01 Impact factor: 7.661
Authors: M F McCann; D E Irwin; L A Walton; B S Hulka; J L Morton; C M Axelrad Journal: Cancer Epidemiol Biomarkers Prev Date: 1992 Jan-Feb Impact factor: 4.254
Authors: Philippe O Van Trappen; Dawn Steele; David G Lowe; Suhail Baithun; Nigel Beasley; Wilko Thiele; Herbert Weich; Jaya Krishnan; John H Shepherd; Michael S Pepper; David G Jackson; Jonathan P Sleeman; Ian J Jacobs Journal: J Pathol Date: 2003-12 Impact factor: 7.996