| Literature DB >> 2194400 |
P P Tamburini1, R N Dreyer, J Hansen, J Letsinger, J Elting, A Gore-Willse, R Dally, R Hanko, D Osterman, M E Kamarck.
Abstract
A rapid sensitive method for the quantification of in vitro HIV-protease activity has been developed on the basis of the endoproteolytic conversion of N-Dns-SQ-NYPIV to N-Dns-SQNY. The use of the N-dansyl group as a fluorescence label was shown to not significantly alter the apparent kinetic parameters for the peptide-enzyme interaction. Using fluorescence detection, the dansylated product and unconverted substrate are detected in a single rapid (3 min) isocratic reverse-phase HPLC separation in quantities as low as 0.2 pmol. The method is highly reproducible and suited to a variety of applications including the analysis of large sample numbers and rigorous enzymological studies.Entities:
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Year: 1990 PMID： 2194400 DOI： 10.1016/0003-2697(90)90095-q
Source DB: PubMed Journal: Anal Biochem ISSN： 0003-2697 Impact factor: 3.365