| Literature DB >> 2194231 |
N Rhodes1, M Company, B Errede.
Abstract
A yeast-Escherichia coli shuttle vector containing the M13 origin of replication has been constructed. This vector allows selection and replication in both Saccharomyces cerevisiae and E. coli, as well as single-stranded packaging from E. coli upon infection with a helper phage. The presence of a polylinker with various unique restriction sites facilitates the cloning of desired genes.Entities:
Mesh:
Year: 1990 PMID: 2194231 DOI: 10.1016/0147-619x(90)90036-c
Source DB: PubMed Journal: Plasmid ISSN: 0147-619X Impact factor: 3.466