Literature DB >> 2193772

Transfection of cultured fish cells RBCF-1 with exogenous oncogene and their resistance to malignant transformation.

K Hayasaka1, M Sato, H Mitani, A Shima.   

Abstract

1. Plasmids bearing the G418-resistant gene neo were transfected into cultured fish cells RBCF-1 by electroporation at an efficiency comparable to that in NIH3T3 cells. 2. Transfection of plasmids bearing both neo and activated human c-Ha-ras into NIH3T3 and RBCF-1 cells resulted in the malignant transformation of the former but not of the latter cells.

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Year:  1990        PMID: 2193772     DOI: 10.1016/0305-0491(90)90387-9

Source DB:  PubMed          Journal:  Comp Biochem Physiol B        ISSN: 0305-0491


  2 in total

1.  Technology and uses of cell cultures from the tissues and organs of bony fish.

Authors:  N C Bols; L E Lee
Journal:  Cytotechnology       Date:  1991-07       Impact factor: 2.058

2.  Improvement of transfection efficiency of epithelioma papulosum cyprini carp cells by modification of cell cycle and use of an optimal promoter.

Authors:  A Rocha; S Ruiz; J M Coll
Journal:  Mar Biotechnol (NY)       Date:  2004-11-04       Impact factor: 3.619

  2 in total

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