[Antibiotic resistance profiles of Acinetobacter species isolated from several clinical samples between 2007-2010].

This study was aimed to investigate the changes in antibiotic resistance profiles of Acinetobacter spp. in our hospital during a four-year period. The study included a total of 465 non-duplicate Acinetobacter spp. isolated from various samples sent from intensive care (n= 274, 58.9%), inpatient (n= 141, 30.3%) and outpatient (n= 49, 10.5%) units of our hospital between 2007 and 2010. Sample distribution was as follows: 184 tracheal aspirates (39.5%), 70 blood (15.3%), 92 (19.8%) wound, 40 urine (8.6%), 24 sputum (5.1%), 22 (4.7%) bronchial lavage and 22 (4.7%) other (catheter tip, cerebrospinal fluid, thorasynthesis material) samples. The isolates were identified as A.baumannii (n= 340, 73.1%), A.lwoffii (n= 64, 13.7%) and Acinetobacter spp. (n= 61, 13.1%). The susceptibility profiles were investigated by Kirby-Bauer disc diffusion method. Overall, the results indicated an increase in resistance against all tested drugs since 2007. A steady increase of resistance from 2007 to 2009, followed by a tendency to decrease in 2010 was also noted for all drugs, except for ceftazidime (CAZ), trimethoprim-sulfomethoxazole (SXT), netilmicin (NET), imipenem (IPM), meropenem (MER) and gentamicin (CN). NET, IPM, cefepime and MER resistance rates increased regularly from 2007 to 2010. CAZ resistance followed a fluctuating course, while CN resistance displayed a decreasing trend since 2009. According to the statistical analyses (X2 and Fisher’s exact test), there was a regular resistance increase between 2007-2009 except for amikacin (AK), SXT and PIP. Resistance rates were also increased for AK and PIP, but only between 2007 and 2009; as well as for piperacillin-tazobactam, ticarcilin-clavulanate, NET, MER and IPM between 2008 and 2009. A significant increase from 2008 to 2010 was observed for NET; and a significant resistance decrease in 2010 was noted for only sultamicillin, cefotaxime, CN and tobramycin (TOB) (p< 0.05). As of 2010, the results indicated high resistance rates against ciprofloxacin [resistance rate (RR): 79%], NET (RR: 60%) and all beta-lactam drugs, including carbapenems (mean RR: 80%). Moreover, there was a progressive increase in resistance to carbapenems and NET, two very important treatment alternatives. Tigecycline (RR: 5.5%), TOB (RR: 19%), CN (RR: 34%) and cefoperazone-sulbactam (RR: 38%) appeared to remain as relatively effective treatment choices. The resistance rates of inpatient and outpatient isolates which were usually lower than those of the intensive care unit isolates, also displayed a noteworthy increase over the past four years. Evidently, pan-resistant Acinetobacter spp. will become a serious health problem in the near future, unless efficient and appropriate precautions are taken.