| Literature DB >> 21931953 |
Kristýna Pimková1, Markéta Bocková, Kateřina Hegnerová, Jiří Suttnar, Jaroslav Cermák, Jiří Homola, Jan E Dyr.
Abstract
The surface plasmon resonance (SPR) biosensor system with dispersionless microfluidics for the direct and label-free detection of a soluble vascular endothelial growth factor receptor (sVEGFR-1) is described. The detection approach takes advantage of an affinity interaction between sVEGFR-1 and its ligand, vascular endothelial growth factor (VEGF-A), which is covalently immobilized on the surface of the SPR sensor. The ability of the immobilized VEGF-A to specifically bind the sVEGFR-1 receptor is demonstrated in a buffer. The detection of sVEGFR-1 in 2% human blood plasma is carried out by using the sequential injection approach. The detection limit of 25 ng/mL is achieved. In addition, we demonstrate that the functional surface of the sensor can be regenerated for repeated use.Entities:
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Year: 2011 PMID： 21931953 DOI： 10.1007/s00216-011-5395-3
Source DB: PubMed Journal: Anal Bioanal Chem ISSN： 1618-2642 Impact factor: 4.142