Laurent Poirel1, Amina Benouda, Constantin Hays, Patrice Nordmann. 1. Service de Bactériologie-Virologie, INSERM U914 Emerging Resistance to Antibiotics, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine Paris Sud, K-Bicêtre, France. laurent.poirel@bct.aphp.fr
Abstract
OBJECTIVES: To analyse the mechanisms responsible for multidrug resistance in three carbapenem-resistant Klebsiella pneumoniae isolates recovered from two hospitalized patients and an outpatient from the same hospital in Rabat, Morocco. METHODS: PCR and sequencing were used to search for β-lactamase genes. Clonal relationships between the isolates were analysed by multilocus sequence typing and PFGE. RESULTS: A history of prior hospitalization in the same ward was determined for two of the three patients. The three isolates of K. pneumoniae belonged to the same sequence type (ST), ST15, and were clonally related. All three isolates carried the bla(NDM-1) gene and co-expressed the extended-spectrum β-lactamases CTX-M-15 and SHV-5, as well as the narrow-spectrum β-lactamases SHV-1, OXA-1, OXA-9 and TEM-1. The bla(NDM-1) gene was located on an ~250 kb non-typeable plasmid co-harbouring the bla(OXA-1) and bla(CTX-M-15) genes. No link with the Indian subcontinent could be established for the three patients. CONCLUSIONS: This work further emphasizes the spread of NDM-1 producers worldwide.
OBJECTIVES: To analyse the mechanisms responsible for multidrug resistance in three carbapenem-resistant Klebsiella pneumoniae isolates recovered from two hospitalized patients and an outpatient from the same hospital in Rabat, Morocco. METHODS: PCR and sequencing were used to search for β-lactamase genes. Clonal relationships between the isolates were analysed by multilocus sequence typing and PFGE. RESULTS: A history of prior hospitalization in the same ward was determined for two of the three patients. The three isolates of K. pneumoniae belonged to the same sequence type (ST), ST15, and were clonally related. All three isolates carried the bla(NDM-1) gene and co-expressed the extended-spectrum β-lactamases CTX-M-15 and SHV-5, as well as the narrow-spectrum β-lactamases SHV-1, OXA-1, OXA-9 and TEM-1. The bla(NDM-1) gene was located on an ~250 kb non-typeable plasmid co-harbouring the bla(OXA-1) and bla(CTX-M-15) genes. No link with the Indian subcontinent could be established for the three patients. CONCLUSIONS: This work further emphasizes the spread of NDM-1 producers worldwide.
Authors: J Fiett; A Baraniak; R Izdebski; I Sitkiewicz; D Żabicka; A Meler; K Filczak; W Hryniewicz; M Gniadkowski Journal: Antimicrob Agents Chemother Date: 2013-11-18 Impact factor: 5.191
Authors: Andreas Schlüter; Patrice Nordmann; Rémy A Bonnin; Yves Millemann; Felix G Eikmeyer; Daniel Wibberg; Alfred Pühler; Laurent Poirel Journal: Antimicrob Agents Chemother Date: 2014-04-21 Impact factor: 5.191