Literature DB >> 2192738

The application of enhanced chemiluminescence to membrane-based nucleic acid detection.

I Durrant1, L C Benge, C Sturrock, A T Devenish, R Howe, S Roe, M Moore, G Scozzafava, L M Proudfoot, T C Richardson.   

Abstract

The ECL gene detection system is a novel, sensitive, non-radioactive system for the detection of nucleic acid hybridized on both nylon and nitrocellulose membranes. It is characterized by direct labeling of probe sequences with horseradish peroxidase combined with an enhanced chemiluminescent (ECL) detection reaction; the light output is captured on blue-light sensitive film. The application of the system to a range of standard molecular biology hybridization techniques is described.

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Year:  1990        PMID: 2192738

Source DB:  PubMed          Journal:  Biotechniques        ISSN: 0736-6205            Impact factor:   1.993


  3 in total

1.  Preparation of horseradish peroxidase-labeled probes.

Authors:  I Durrant; T Stone
Journal:  Mol Biotechnol       Date:  1996-08       Impact factor: 2.695

2.  Hybridization of horseradish peroxidase-labeled probes and detection by enhanced chemiluminescence.

Authors:  T Stone; I Durrant
Journal:  Mol Biotechnol       Date:  1996-08       Impact factor: 2.695

3.  Immobilization of polynucleotides on magnetic particles. Factors influencing hybridization efficiency.

Authors:  P J Day; P S Flora; J E Fox; M R Walker
Journal:  Biochem J       Date:  1991-09-15       Impact factor: 3.857

  3 in total

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