Literature DB >> 2192576

An alternative quenched fluorescence substrate for Pz-peptidase.

U Tisljar1, C G Knight, A J Barrett.   

Abstract

7-Methoxycoumarin-3-carboxylyl-Pro-Leu-Gly-Pro-D-Lys(2,4-dinitr oph enyl) is introduced as a new quenched fluorescence substrate for assaying Pz-peptidase (also known as soluble metallo-endopeptidase and endo-oligopeptidase). The value of Km for partially purified Pz-peptidase from rat muscle was 8.6 microM. High protein concentrations did not interfere with the assay, so that for the first time continuous assays of Pz-peptidase in crude tissue extracts became possible.

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Year:  1990        PMID： 2192576     DOI： 10.1016/0003-2697(90)90582-t

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  16 in total

1.  Development and characterization of novel potent and stable inhibitors of endopeptidase EC 3.4.24.15.

Authors:  C N Shrimpton; G Abbenante; R A Lew; I Smith
Journal:  Biochem J       Date:  2000-01-15       Impact factor: 3.857

2.  Fluorimetric assay of the neurotensin-degrading metalloendopeptidase, endopeptidase 24.16.

Authors:  P Dauch; H Barelli; J P Vincent; F Checler
Journal:  Biochem J       Date:  1991-12-01       Impact factor: 3.857

3.  Role of bacterial peptidase F inferred by statistical analysis and further experimental validation.

Authors:  Liliana Lopez Kleine; Véronique Monnet; Christine Pechoux; Alain Trubuil
Journal:  HFSP J       Date:  2008-01-07

4.  Thimet oligopeptidase (EC 3.4.24.15): the same by any name?

Authors:  A J Barrett
Journal:  Biochem J       Date:  1991-07-01       Impact factor: 3.857

5.  The exquisite structure and reaction mechanism of bacterial Pz-peptidase A toward collagenous peptides: X-ray crystallographic structure analysis of PZ-peptidase a reveals differences from mammalian thimet oligopeptidase.

Authors:  Akio Kawasaki; Hiroaki Nakano; Allin Hosokawa; Toru Nakatsu; Hiroaki Kato; Kunihiko Watanabe
Journal:  J Biol Chem       Date:  2010-09-03       Impact factor: 5.157

6.  Synthetic inhibitors of endopeptidase EC 3.4.24.15: potency and stability in vitro and in vivo.

Authors:  R A Lew; F Tomoda; R G Evans; L Lakat; J H Boublik; L A Pipolo; A I Smith
Journal:  Br J Pharmacol       Date:  1996-07       Impact factor: 8.739

7.  Rat thimet oligopeptidase: large-scale expression in Escherichia coli and characterization of the recombinant enzyme.

Authors:  N McKie; P M Dando; M A Brown; A J Barrett
Journal:  Biochem J       Date:  1995-07-01       Impact factor: 3.857

8.  Thimet oligopeptidase specificity: evidence of preferential cleavage near the C-terminus and product inhibition from kinetic analysis of peptide hydrolysis.

Authors:  C G Knight; P M Dando; A J Barrett
Journal:  Biochem J       Date:  1995-05-15       Impact factor: 3.857

9.  Potent inhibition of endopeptidase 24.16 and endopeptidase 24.15 by the phosphonamide peptide N-(phenylethylphosphonyl)-Gly-L-Pro-L-aminohexanoic acid.

Authors:  H Barelli; V Dive; A Yiotakis; J P Vincent; F Checler
Journal:  Biochem J       Date:  1992-10-15       Impact factor: 3.857

10.  Thimet oligopeptidase: similarity to 'soluble angiotensin II-binding protein' and some corrections to the published amino acid sequence of the rat testis enzyme.

Authors:  N McKie; P M Dando; N D Rawlings; A J Barrett
Journal:  Biochem J       Date:  1993-10-01       Impact factor: 3.857
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