Literature DB >> 21922490

Electrokinetic DNA transport in 20 nm-high nanoslits: evidence for movement through a wall-adsorbed.

Oscar Castillo-Fernandez1, Georgette B Salieb-Beugelaar, Jan W van Nieuwkasteele, Johan G Bomer, Martin Arundell, Josep Samitier, Albert van den Berg, Jan C T Eijkel.   

Abstract

The electrokinetic transport behavior of λ-DNA (48 kbp) in 20 nm-high fused-silica nanoslits in the presence of short-chain PVP is investigated. Mobility and video data show a number of phenomena that are typical of DNA transport through gels or polymer solutions, thus indicative of rigid migration obstacles in the DNA pathway. Calculations show that a several nanometer thin layer of wall-adsorbed PVP ('nano-gel') can provide such a rigid obstacle matrix to the DNA. Such ultrathin wall-adsorbed polymer layers represent a new type of matrix for electrokinetic DNA separation.
Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

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Year:  2011        PMID： 21922490     DOI： 10.1002/elps.201100278

Source DB:  PubMed          Journal:  Electrophoresis        ISSN： 0173-0835            Impact factor:   3.535


  8 in total

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Review 2.  Beyond gel electrophoresis: microfluidic separations, fluorescence burst analysis, and DNA stretching.

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Journal:  Anal Chem       Date:  2012-12-24       Impact factor: 6.986

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6.  Spatial confinement induces hairpins in nicked circular DNA.

Authors:  Aleksandre Japaridze; Enzo Orlandini; Kathleen Beth Smith; Lucas Gmür; Francesco Valle; Cristian Micheletti; Giovanni Dietler
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7.  Label-Free Identification of Single Mononucleotides by Nanoscale Electrophoresis.

Authors:  Junseo Choi; Zheng Jia; Ramin Riahipour; Collin J McKinney; Charuni A Amarasekara; Kumuditha M Weerakoon-Ratnayake; Steven A Soper; Sunggook Park
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Review 8.  Capillary electrophoresis applied to DNA: determining and harnessing sequence and structure to advance bioanalyses (2009-2014).

Authors:  Brandon C Durney; Cassandra L Crihfield; Lisa A Holland
Journal:  Anal Bioanal Chem       Date:  2015-05-03       Impact factor: 4.142
  8 in total

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