BACKGROUND AND AIM: In Barrett's esophagus (BE), the normal esophageal squamous epithelium is replaced with a specialized metaplastic columnar epithelium. BE is a premalignant lesion that can progress to esophageal adenocarcinoma (EAC). Currently, there are no early molecular indicators that would predict progression from BE to EAC. As the only permanent residents of the epithelium, stem cells have been implicated in this metaplastic progression. The aim of the present study was to determine the expression of doublecortin and CaM kinase-like-1 (DCAMKL-1) and other putative gastrointestinal stem cell markers in normal esophageal mucosa (NEM), BE, and EAC. METHODS: Human NEM, BE, EAC, and multitissue microarrays were analyzed for DCAMKL-1, and immunohistochemically scored based on staining intensity and tissue involvement, with epithelia and stroma scored separately. Total RNA isolated from BE and paired NEM was subjected to real-time reverse-transcription-polymerase chain reaction analysis for DCAMKL-1, leucine-rich repeat-containing G-protein-coupled receptor (LGR5), and Musashi-1 (Msi-1) mRNA expression. RESULTS: DCAMKL-1 was minimally expressed in squamous NEM, but increased in BE (with and without dysplasia) and EAC tissues. In EAC, we found increased stromal DCAMKL-1 staining compared to adjacent epithelia. Within the submucosa of dysplastic BE tissues, an increase in the endothelial cell expression of DCAMKL-1 was observed. Finally, an upregulation of DCAMKL-1, LGR5, and Msi-1 mRNA was seen in BE compared to squamous NEM. CONCLUSIONS: In the present study, we report the progressive increase of DCAMKL-1 expression in BE from dysplasia to EAC. Furthermore, there was an increase in putative stem cell markers DCAMKL-1, LGR5, and Msi-1 mRNA. Taken together, these data suggest that the regulation of resident stem cells might play an important role in the progression of BE to EAC.
BACKGROUND AND AIM: In Barrett's esophagus (BE), the normal esophageal squamous epithelium is replaced with a specialized metaplastic columnar epithelium. BE is a premalignant lesion that can progress to esophageal adenocarcinoma (EAC). Currently, there are no early molecular indicators that would predict progression from BE to EAC. As the only permanent residents of the epithelium, stem cells have been implicated in this metaplastic progression. The aim of the present study was to determine the expression of doublecortin and CaM kinase-like-1 (DCAMKL-1) and other putative gastrointestinal stem cell markers in normal esophageal mucosa (NEM), BE, and EAC. METHODS:Human NEM, BE, EAC, and multitissue microarrays were analyzed for DCAMKL-1, and immunohistochemically scored based on staining intensity and tissue involvement, with epithelia and stroma scored separately. Total RNA isolated from BE and paired NEM was subjected to real-time reverse-transcription-polymerase chain reaction analysis for DCAMKL-1, leucine-rich repeat-containing G-protein-coupled receptor (LGR5), and Musashi-1 (Msi-1) mRNA expression. RESULTS:DCAMKL-1 was minimally expressed in squamous NEM, but increased in BE (with and without dysplasia) and EAC tissues. In EAC, we found increased stromal DCAMKL-1 staining compared to adjacent epithelia. Within the submucosa of dysplastic BE tissues, an increase in the endothelial cell expression of DCAMKL-1 was observed. Finally, an upregulation of DCAMKL-1, LGR5, and Msi-1 mRNA was seen in BE compared to squamous NEM. CONCLUSIONS: In the present study, we report the progressive increase of DCAMKL-1 expression in BE from dysplasia to EAC. Furthermore, there was an increase in putative stem cell markers DCAMKL-1, LGR5, and Msi-1 mRNA. Taken together, these data suggest that the regulation of resident stem cells might play an important role in the progression of BE to EAC.
Authors: Sripathi M Sureban; Randal May; Stan A Lightfoot; Aimee B Hoskins; Megan Lerner; Daniel J Brackett; Russell G Postier; Rama Ramanujam; Altaf Mohammed; Chinthalapally V Rao; James H Wyche; Shrikant Anant; Courtney W Houchen Journal: Cancer Res Date: 2011-02-01 Impact factor: 12.701
Authors: Randal May; Sripathi M Sureban; Stan A Lightfoot; Aimee B Hoskins; Daniel J Brackett; Russell G Postier; Rama Ramanujam; Chinthalapally V Rao; James H Wyche; Shrikant Anant; Courtney W Houchen Journal: Am J Physiol Gastrointest Liver Physiol Date: 2010-06-03 Impact factor: 4.052
Authors: C Ell; A May; L Gossner; O Pech; E Günter; G Mayer; R Henrich; M Vieth; H Müller; G Seitz; M Stolte Journal: Gastroenterology Date: 2000-04 Impact factor: 22.682
Authors: Brechtje A Grotenhuis; Winand N M Dinjens; Bas P L Wijnhoven; Petra Sonneveld; Andrea Sacchetti; Patrick F Franken; Herman van Dekken; Hugo W Tilanus; J Jan B van Lanschot; Riccardo Fodde Journal: J Pathol Date: 2010-08 Impact factor: 7.996
Authors: James Cronin; Elizabeth McAdam; Antonios Danikas; Chris Tselepis; Paul Griffiths; John Baxter; Linzi Thomas; James Manson; Gareth Jenkins Journal: Am J Gastroenterol Date: 2010-12-14 Impact factor: 10.864
Authors: Jonathan Mendelson; Shumei Song; Ying Li; Dipen M Maru; Bibhuti Mishra; Marta Davila; Wayne L Hofstetter; Lopa Mishra Journal: Cancer Date: 2011-02-08 Impact factor: 6.860
Authors: Douglas K Rex; Oscar W Cummings; Michael Shaw; Mark D Cumings; Roy K H Wong; Raj S Vasudeva; Donal Dunne; Emad Y Rahmani; Debra J Helper Journal: Gastroenterology Date: 2003-12 Impact factor: 22.682
Authors: Burkhard H A von Rahden; Stefan Kircher; Maria Lazariotou; Christoph Reiber; Luisa Stuermer; Christoph Otto; Christoph T Germer; Martin Grimm Journal: J Exp Clin Cancer Res Date: 2011-02-23
Authors: Stuart A C McDonald; Danielle Lavery; Nicholas A Wright; Marnix Jansen Journal: Nat Rev Gastroenterol Hepatol Date: 2014-11-04 Impact factor: 46.802
Authors: Moritz Middelhoff; C Benedikt Westphalen; Yoku Hayakawa; Kelley S Yan; Michael D Gershon; Timothy C Wang; Michael Quante Journal: Am J Physiol Gastrointest Liver Physiol Date: 2017-07-06 Impact factor: 4.052
Authors: Raphael Schellnegger; Anne Quante; Susanne Rospleszcz; Martina Schernhammer; Bettina Höhl; Moritz Tobiasch; Agnieszka Pastula; Anna Brandtner; Julian A Abrams; Konstantin Strauch; Roland M Schmid; Michael Vieth; Timothy C Wang; Michael Quante Journal: Cancer Prev Res (Phila) Date: 2016-11-02
Authors: Wanglong Qiu; Helen E Remotti; Sophia M Tang; Elizabeth Wang; Lily Dobberteen; Ayman Lee Youssof; Joo Hee Lee; Edwin C Cheung; Gloria H Su Journal: Cancer Lett Date: 2018-03-08 Impact factor: 8.679