Tobias K Boehm1, Sebastian G Ciancio. 1. College of Dental Medicine, Western University of Health Sciences, 309 East Second Street, Pomona, CA 91766, USA. tboehm@westernu.edu
Abstract
BACKGROUND AND OBJECTIVE: Commercially available photodynamic therapy for periodontal diseases utilizes methylene blue as a photosensitizer. Here we propose a novel photosensitizer dye, indocyanine green (ICG), because it can be readily activated by commercially available dental 810 nm diode lasers and has an established safety record as an intravascular agent in cardiac imaging and ophthalmologic photodynamic therapy. Therefore, we aim to characterize ICG uptake and killing of key periodontal pathogens to explore its potential as a periodontal photodynamic therapy agent. MATERIALS AND METHODS: We tested ICG uptake by spectroscopy in Porphyromonas gingivalis 381 and Aggregatibacter actinomycetemcomitans, in addition to Escherichia coli DH5alpha and a human gingival epithelial cell line, HepG, in relation to ICG dose and exposure time. We then measured killing of bacteria by determining viable bacteria counts before and after exposure to ICG and 810 nm diode laser light (0-0.5 W output settings, 0-5 seconds). ICG was also applied to extracted, restored teeth, and the teeth inspected visually for staining after rinsing with saline. RESULTS: We found rapid and significant uptake of indocyanine green into P. gingivalis 381 and A. actinomycetemcomitans 67, compared to E. coli DH5alpha and HepG gingival cell line. This correlated with significant killing of strains 381 and 67 compared to E.coli, with less than 10% survival. ICG does not appear to stain tooth surfaces and materials except calculus. CONCLUSION: ICG combined with an 810 nm diode laser may be useful as a photodynamic adjunct for reduction of bacterial load in periodontal pockets.
BACKGROUND AND OBJECTIVE: Commercially available photodynamic therapy for periodontal diseases utilizes methylene blue as a photosensitizer. Here we propose a novel photosensitizer dye, indocyanine green (ICG), because it can be readily activated by commercially available dental 810 nm diode lasers and has an established safety record as an intravascular agent in cardiac imaging and ophthalmologic photodynamic therapy. Therefore, we aim to characterize ICG uptake and killing of key periodontal pathogens to explore its potential as a periodontal photodynamic therapy agent. MATERIALS AND METHODS: We tested ICG uptake by spectroscopy in Porphyromonas gingivalis 381 and Aggregatibacter actinomycetemcomitans, in addition to Escherichia coli DH5alpha and a human gingival epithelial cell line, HepG, in relation to ICG dose and exposure time. We then measured killing of bacteria by determining viable bacteria counts before and after exposure to ICG and 810 nm diode laser light (0-0.5 W output settings, 0-5 seconds). ICG was also applied to extracted, restored teeth, and the teeth inspected visually for staining after rinsing with saline. RESULTS: We found rapid and significant uptake of indocyanine green into P. gingivalis 381 and A. actinomycetemcomitans 67, compared to E. coli DH5alpha and HepG gingival cell line. This correlated with significant killing of strains 381 and 67 compared to E.coli, with less than 10% survival. ICG does not appear to stain tooth surfaces and materials except calculus. CONCLUSION:ICG combined with an 810 nm diode laser may be useful as a photodynamic adjunct for reduction of bacterial load in periodontal pockets.
Authors: Yoshiko Masuda; Hiroshi Sakagami; Masashi Horiike; Hiroshi Kadokura; Takahide Yamasaki; Perry R Klokkevold; Henry H Takei; Satoshi Yokose Journal: In Vivo Date: 2018 Jul-Aug Impact factor: 2.155